Huangfu Xiaoxia, Shen Yang, Yang Anzi, Liu Lixiao, Luo Wen, Zhao Wenbo
National and Local Joint Engineering Research Center of Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing, 210023, China.
National and Local Joint Engineering Research Center of Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing, 210023, China.
Colloids Surf B Biointerfaces. 2020 Jul;191:110984. doi: 10.1016/j.colsurfb.2020.110984. Epub 2020 Mar 19.
Developing monitoring technique for alkaline phosphatase (ALP) is crucial due to the important role it plays in living cells. Here, a kind of biocompatible glutathione-modified CuGaS/ZnS quantum dots (GSH-CGS/ZnS QDs) was used as a fluorescent substance and then fabricated "turn-off" fluorescent biosensor for detection of ALP by help of inner filter effect (IFE). Firstly, we prepared CuGaS/ZnS (CGS/ZnS) QDs using solvothermal method and explored the efficient ligand (GSH) exchanges strategy for transferring oil-soluble CGS/ZnS QDs to aqueous phase. More importantly, we also explored the potential biological applications of the nanohybrid QDs. The obtained GSH-CGS/ZnS QDs emitted strong yellow fluorescence with the maximum excitation (400 nm) and emission (601 nm). Then, GSH-CGS/ZnS QDs were mixed with p-nitrophenylphosphate (PNPP) and ALP. PNPP could be hydrolyzed to p-nitrophenol (PNP) by help of catalysis of ALP, and the excitation spectrum of the GSH-CGS/ZnS QDs overlapped well with the absorption spectrum of PNP, so the fluorescence of GSH-CGS/ZnS QDs was initially quenched via the so-called "IFE". Finally, a novel "turn-off" biosensor for sensitive detection of ALP in the range of 0.05-10 U L (R = 0.98) with a detection limit of 0.01 U L was successfully obtained. Results indicated that I-III-VI nanocrystals have great potential for their promising biomedical application.
由于碱性磷酸酶（ALP）在活细胞中发挥的重要作用，开发其监测技术至关重要。在此，一种生物相容性的谷胱甘肽修饰的CuGaS/ZnS量子点（GSH-CGS/ZnS QDs）被用作荧光物质，并借助内滤效应（IFE）构建了用于检测ALP的“关断型”荧光生物传感器。首先，我们采用溶剂热法制备了CuGaS/ZnS（CGS/ZnS）量子点，并探索了将油溶性CGS/ZnS量子点转移到水相的高效配体（GSH）交换策略。更重要的是，我们还探索了纳米杂化量子点的潜在生物应用。所获得的GSH-CGS/ZnS量子点在最大激发波长（400nm）和发射波长（601nm）处发出强烈的黄色荧光。然后，将GSH-CGS/ZnS量子点与对硝基苯磷酸酯（PNPP）和ALP混合。在ALP的催化作用下，PNPP可水解为对硝基苯酚（PNP），且GSH-CGS/ZnS量子点的激发光谱与PNP的吸收光谱有很好的重叠，因此GSH-CGS/ZnS量子点的荧光最初通过所谓的“IFE”被猝灭。最终，成功获得了一种新型的“关断型”生物传感器，用于在0.05 - 10 U/L范围内灵敏检测ALP（R = 0.98），检测限为0.01 U/L。结果表明，I-III-VI族纳米晶体因其有前景的生物医学应用而具有巨大潜力。
