Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya, Japan.
Exploratory Research Center on Life and Living Systems (ExCELLS), National Institutes of Natural Sciences, Okazaki, Japan.
Methods Mol Biol. 2020;2132:21-28. doi: 10.1007/978-1-0716-0430-4_3.
Animal leguminous-type (L-type) lectins, including ERGIC-53 and VIP36 are responsible for intracellular transport and quality control of N-linked glycoproteins in the early secretory pathway. These lectins possess the carbohydrate recognition domain (CRD), which recognizes high-mannose-type glycans in a Ca-dependent manner. Here we describe the procedures involved in bacterial overproduction and purification of the CRDs of the animal L-type lectins.
动物豆科型 (L 型) 凝集素，包括 ERGIC-53 和 VIP36，负责在早期分泌途径中对 N-连接糖蛋白进行细胞内运输和质量控制。这些凝集素具有碳水化合物识别结构域 (CRD)，以 Ca 依赖性方式识别高甘露糖型聚糖。在这里，我们描述了细菌过量生产和纯化动物 L 型凝集素的 CRD 所涉及的程序。
