Animal Research Centre, Directorate General of Veterinary Services, Royal Court Affairs, P.O. Box: 64, P.C: 111, Muscat, Oman.
Animal Research Centre, Directorate General of Veterinary Services, Royal Court Affairs, P.O. Box: 64, P.C: 111, Muscat, Oman.
Theriogenology. 2020 Jul 15;151:112-118. doi: 10.1016/j.theriogenology.2020.04.017. Epub 2020 Apr 16.
This study was conducted to develop simple superovulation protocols for dromedary camels using eCG. In experiment 1, camels received either 1000, 2000, 3000, 4000, 5000 or 6000 IU eCG. In experiment 2, camels received either 400 mg FSH (Folltropin-V) twice-daily over 5 days or 3000 IU eCG. In experiment 3, camels received 3000 IU eCG either at 2, 3, 4 or 5 days after ovulation induction. Ovarian response and embryo yield were evaluated in all experiments and embryos collected from camels treated with FSH and eCG were transferred to recipients to examine pregnancy rates. The mean number of ovulations (12.6 ± 1.5 and 13.3 ± 1.2 vs 3.4 ± 0.3, 6.2 ± 0.6 and 9.3 ± 1.0, respectively) and transferable embryos (4.6 ± 1.3 and 4.8 ± 1.0 vs 1.6 ± 0.2, 2.2 ± 0.4 and 1.1 ± 0.4, respectively) with 3000 and 4000 IU eCG doses were higher compared to 1000, 2000 and 6000 IU eCG doses (P < 0.05). Doses of 5000 and 6000 IU eCG resulted in a higher number of unovulatory follicles than other doses (P < 0.05). The FSH treatment resulted in higher number of ovulatory follicles (21.8 ± 1.3 vs 14.8 ± 1.7) and ovulations (18.5 ± 1.1 vs 13.9 ± 1.4) compared to eCG (P < 0.05). However, the number of transferable embryos and pregnancy rates were similar in these treatments. The timing of eCG treatment after ovulation induction did not affect the number of ovulatory follicles and transferable embryos but eCG treatment at 5 days after ovulation induction reduced the number of ovulations (P < 0.05). In conclusion, the optimal dose of eCG to induce superovulation is 3000-4000 IU and it produces a comparable embryo yield to FSH, and can be administered at 2-4 days after ovulation induction.
本研究旨在开发使用 eCG 对单峰驼进行简单超排卵的方案。在实验 1 中，骆驼接受了 1000、2000、3000、4000、5000 或 6000IU eCG。在实验 2 中，骆驼接受了 400mgFSH（Folltropin-V）每日两次，持续 5 天或 3000IU eCG。在实验 3 中，骆驼在排卵诱导后 2、3、4 或 5 天接受 3000IU eCG。在所有实验中评估了卵巢反应和胚胎产量，并将从接受 FSH 和 eCG 治疗的骆驼中收集的胚胎转移到受体中，以检查妊娠率。用 3000 和 4000IU eCG 剂量的平均排卵数（分别为 12.6±1.5 和 13.3±1.2 与 3.4±0.3、6.2±0.6 和 9.3±1.0）和可转移胚胎数（分别为 4.6±1.3 和 4.8±1.0 与 1.6±0.2、2.2±0.4 和 1.1±0.4）高于 1000、2000 和 6000IU eCG 剂量（P<0.05）。5000 和 6000IU eCG 剂量导致未排卵卵泡的数量高于其他剂量（P<0.05）。与 eCG 相比，FSH 处理导致更多的排卵卵泡（21.8±1.3 与 14.8±1.7）和排卵（18.5±1.1 与 13.9±1.4）（P<0.05）。然而，这些处理中的可转移胚胎数量和妊娠率相似。排卵诱导后 eCG 治疗的时间不影响排卵卵泡和可转移胚胎的数量，但排卵诱导后 5 天进行 eCG 治疗会减少排卵次数（P<0.05）。总之，诱导超排卵的最佳 eCG 剂量为 3000-4000IU，其胚胎产量与 FSH 相当，可在排卵后 2-4 天给药。
