Department of Engineering, University of South Australia, Adelaide, SA 5095, Australia.
Department of Renal Medicine, Flinders Medical Centre, Flinders University, Bedford Park, SA 5042, Australia.
Int J Mol Sci. 2020 Apr 22;21(8):2963. doi: 10.3390/ijms21082963.
Exogenous administration of hexaminolevulinate (HAL) induces fluorescent protoporphyrin IX (PpIX) accumulation preferentially in cancer cells. However, the PpIX fluorescence intensities between noncancer and cancer cells are highly variable. The contrast between cancer and noncancer cells may be insufficient to reliably discriminate, especially at the single cell level in cancer diagnostics. This study examines the use of the chemical adjuvants dimethylsulphoxide (DMSO) or deferoxamine (DFO) to enhance the HAL induced PpIX accumulation in cancer cells. Our results showed that in some of the incubation conditions tested, the addition of DFO with HAL significantly increased PpIX 21 fluorescence of adherent monolayer cancer cells, but this was never the case for cells in suspension. Permeabilisation with DMSO did not increase PpIX fluorescence. Cell-to-cell interaction may well play an important role in the PpIX accumulation when suspended cells are treated in HAL and adjuvant chemicals.
外源性给予六氨基己酸（HAL）可诱导荧光原卟啉 IX（PpIX）优先在癌细胞中积累。然而，非癌细胞和癌细胞之间的 PpIX 荧光强度差异很大。癌细胞和非癌细胞之间的对比度可能不足以进行可靠的区分，尤其是在癌症诊断的单细胞水平上。本研究探讨了使用化学佐剂二甲基亚砜（DMSO）或去铁胺（DFO）来增强 HAL 诱导的癌细胞中 PpIX 的积累。我们的结果表明，在测试的一些孵育条件下，DFO 与 HAL 的联合使用可显著增加贴壁单层癌细胞中 PpIX 21 的荧光强度，但悬浮细胞的情况却并非如此。DMSO 的透化作用不会增加 PpIX 荧光强度。当悬浮细胞用 HAL 和佐剂化学物质处理时，细胞间相互作用可能在 PpIX 积累中发挥重要作用。
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