光生物调节作用对牙髓细胞产生和释放的血管生成蛋白的影响。
Photobiomodulation effect on angiogenic proteins produced and released by dental pulp cells.
机构信息
Department of Pediatric Dentistry, University of Sacred Heart, Rua Irmã Arminda, 10-50, Bauru, São Paulo, 17011-160, Brazil.
Department of Pediatric Dentistry, Orthodontics, and Public Health, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil.
出版信息
Clin Oral Investig. 2020 Dec;24(12):4343-4354. doi: 10.1007/s00784-020-03298-1. Epub 2020 Apr 24.
OBJECTIVE
To verify the photobiomodulation effect on angiogenic proteins produced and released by dental human pulpal fibroblasts (HPFs).
MATERIAL AND METHODS
HPFs were irradiated with 660-nm low-level laser at fluences of 2.5 J/cm and 3.7 J/cm. The control group was not irradiated. MTT, crystal violet, and ELISA assays respectively verified viability, proliferation, and angiogenic protein (supernatant/lysate) at 6 h, 12 h, and 24 h after photobiomodulation. Capillary-like structure formation assay verified functional role. Two-way ANOVA/Tukey's test and ANOVA/Bonferroni's multiple comparisons test respectively verified cell viability/proliferation and intragroup and intergroup comparisons of protein synthesis (p < 0.05).
RESULTS
Irradiated and non-irradiated HPFs showed statistically similar cell viability and proliferation pattern. Intragroup comparisons showed similar patterns of protein synthesis for all groups: VEGF-A, VEGF-C, and vascular endothelial growth factor receptor 1 (VEGFR1) increased significantly in the supernatant, while FGF-2 and VEGF-A increased significantly in the lysate. The lower fluence significantly increased BMP-9 (6 h) in the supernatant and VEGFR1 (6 h and 12 h) and VEGF-D (24 h) in the lysate, while the higher fluence significantly increased BMP-9 (6 h) in the supernatant and VEGFR1 (12 h) in the lysate. Regardless of the time, both fluences statistically downregulated placental growth factor (PLGF) and PDGF secretion. Both fluences statistically decreased VEGF-A secretion (24 h) and PLGF production (6 h).
CONCLUSION
Photobiomodulation produced stimulatory effects on angiogenic protein secretion by pulp fibroblasts. In terms of photobiomodulation, over time, both fluences significantly increased the secretion of VEGF-A, VEGF-C, and VEGFR1 and significantly upregulated BMP-9 (6 h) in the supernatant; for capillary-like structure formation, the fluence of 2.5 J/cm was better than the fluence of 3.7 J/cm.
CLINICAL RELEVANCE
This study results addressed effective photobiomodulation parameters tailored for pulp angiogenesis.
目的
验证低水平 660nm 激光对牙髓人成纤维细胞（HPF）产生和释放的血管生成蛋白的光生物调节作用。
材料与方法
将 HPF 用 2.5 J/cm 和 3.7 J/cm 的 660nm 低水平激光照射。对照组未照射。分别在光生物调节后 6、12 和 24 小时用 MTT、结晶紫和 ELISA 测定细胞活力、增殖和血管生成蛋白（上清液/裂解物）。毛细血管样结构形成测定验证了功能作用。分别用双向方差分析/Tukey 检验和方差分析/ Bonferroni 多重比较检验验证细胞活力/增殖和蛋白合成的组内和组间比较（p<0.05）。
结果
照射和未照射的 HPF 显示出统计学上相似的细胞活力和增殖模式。组内比较显示所有组的蛋白合成模式相似：VEGF-A、VEGF-C 和血管内皮生长因子受体 1（VEGFR1）在上清液中显著增加，而 FGF-2 和 VEGF-A 在上清液和裂解物中显著增加。较低的剂量在下清液中显著增加 BMP-9（6 小时）和在裂解物中显著增加 VEGFR1（6 小时和 12 小时）和 VEGF-D（24 小时），而较高的剂量在上清液中显著增加 BMP-9（6 小时）和在裂解物中显著增加 VEGFR1（12 小时）。无论时间如何，两种剂量均统计学地下调胎盘生长因子（PLGF）和 PDGF 的分泌。两种剂量均统计学地下调 VEGF-A 的分泌（24 小时）和 PLGF 的产生（6 小时）。
结论
光生物调节对牙髓成纤维细胞的血管生成蛋白分泌产生了刺激作用。就光生物调节而言，随着时间的推移，两种剂量均显著增加了上清液中 VEGF-A、VEGF-C 和 VEGFR1 的分泌，并在上清液中显著上调了 BMP-9（6 小时）；对于毛细血管样结构形成，2.5 J/cm 的剂量优于 3.7 J/cm 的剂量。
临床相关性
本研究结果为牙髓血管生成提供了定制的有效光生物调节参数。
Zotero 插件