钙调蛋白的大规模制备。
Large scale preparation of calmodulin.
作者信息
Newton D L, Krinks M H, Kaufman J B, Shiloach J, Klee C B
机构信息
Laboratory of Biochemistry, National Cancer Institute, Bethesda, MD 20892.
出版信息
Prep Biochem. 1988;18(3):247-59. doi: 10.1080/00327488808062527.
A rapid large scale purification procedure based on three conventional purification steps, ammonium sulfate fractionation, DEAE cellulose and hydroxylapaptite chromotography yields gram amounts of calmodulin. The protein is more than 98% pure by SDS gel electrophoresis and amino acid composition. It is free of contaminating EGTA or EDTA and the omission of heat treatment or denaturing solvents during the preparation avoids possible denaturation of the protein and minimizes partial proteolysis.
基于硫酸铵分级沉淀、DEAE纤维素和羟基磷灰石色谱这三个传统纯化步骤的快速大规模纯化程序可产生克级量的钙调蛋白。通过SDS凝胶电泳和氨基酸组成分析，该蛋白质的纯度超过98%。它不含污染性的乙二醇双四乙酸（EGTA）或乙二胺四乙酸（EDTA），并且在制备过程中省略热处理或变性溶剂可避免蛋白质可能的变性，并将部分蛋白水解降至最低。
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