基于纸张的恒电位电致化学发光传感平台,以黑磷作为发光体,并由钙钛矿太阳能电池提供能量。
Paper-Based Constant Potential Electrochemiluminescence Sensing Platform with Black Phosphorus as a Luminophore Enabled by a Perovskite Solar Cell.
机构信息
School of Chemistry and Chemical Engineering, University of Jinan, Jinan, Shandong 250022, PR China.
Shandong Provincial Key Laboratory of Preparation and Measurement of Building Materials, University of Jinan, Jinan, Shandong 250022, PR China.
出版信息
Anal Chem. 2020 May 19;92(10):6822-6826. doi: 10.1021/acs.analchem.0c01033. Epub 2020 May 10.
Exploring efficient luminophores in the electrochemiluminescence (ECL) system is highly desired to pursue a sensitive ECL sensing platform. Herein, the black phosphorus nanosheets (BP NSs) with excellent ECL properties are investigated and serve as the luminophore with the coreactant of peroxydisulfate (SO) solution. Moreover, owing to the overlapping of emission and absorbance spectra, effective resonance energy transfer (RET) is realized between the BP NSs and the introduced Au nanoparticles. In order to achieve the portable and miniaturized developing trends for the paper-based ECL sensing platform, a paper-based perovskite solar cell (PSC) device is designed to act as the power source to replace the commonly utilized expensive and cumbersome electrochemical workstation. Benefiting from that, a PSC driven paper-based constant potential ECL-RET sensing platform is constructed, thereby realizing sensitive microRNAs (miRNAs) detection. What's more, to attain the preferable analytical performance, the duplex-specific nuclease (DSN) is also introduced to assist the target recycling signal amplification strategy. Based on this, highly sensitive detection of miRNA-107 with a range from 0.1 pM to 15 nM is achieved by this designed sensing platform. Most importantly, this work not only pioneers a precedent for developing a high-sensitivity PSC triggered ECL sensing platform but also explores the application prospect of BP nanomaterial in the field of bioanalysis.
探索电化学发光 (ECL) 体系中高效的发光体,以追求高灵敏度的 ECL 传感平台是非常有必要的。在此,研究了具有优异 ECL 性能的黑磷纳米片 (BP NSs),并将其作为发光体与过二硫酸盐 (SO) 溶液的共反应物。此外,由于发射光谱和吸收光谱的重叠,在 BP NSs 和引入的金纳米粒子之间实现了有效的共振能量转移 (RET)。为了实现基于纸的 ECL 传感平台的便携化和小型化发展趋势,设计了一种基于纸的钙钛矿太阳能电池 (PSC) 器件作为电源,以替代常用的昂贵且笨重的电化学工作站。得益于这一点,构建了一个由 PSC 驱动的基于纸的恒电位 ECL-RET 传感平台,从而实现了对微 RNA (miRNA) 的灵敏检测。更重要的是,为了获得更好的分析性能,还引入了双链特异性核酸酶 (DSN) 以辅助目标循环信号放大策略。基于此,该设计的传感平台实现了对 miRNA-107 的高灵敏度检测,检测范围从 0.1 pM 到 15 nM。最重要的是,这项工作不仅开创了开发高灵敏度 PSC 触发 ECL 传感平台的先例,而且还探索了 BP 纳米材料在生物分析领域的应用前景。