Department of Biological and Environmental Engineering, College of Agriculture and Life Sciences, Cornell University, Ithaca, New York, United States of America.
Department of Civil and Environmental Engineering, McCormick School of Engineering and Applied Science, Northwestern University, Evanston, Illinois, United States of America.
PLoS Comput Biol. 2020 May 8;16(5):e1007841. doi: 10.1371/journal.pcbi.1007841. eCollection 2020 May.
Light is one of the factors that can play a role in bacterial infiltration into leafy greens by keeping stomata open and providing photosynthetic products for microorganisms. We model chemotactic transport of bacteria within a leaf tissue in response to photosynthesis occurring within plant mesophyll. The model includes transport of carbon dioxide, oxygen, bicarbonate, sucrose/glucose, bacteria, and autoinducer-2 within the leaf tissue. Biological processes of carbon fixation in chloroplasts, and respiration in mitochondria of the plant cells, as well as motility, chemotaxis, nutrient consumption and communication in the bacterial community are considered. We show that presence of light is enough to boost bacterial chemotaxis through the stomatal opening and toward photosynthetic products within the leaf tissue. Bacterial chemotactic ability is a major player in infiltration, and plant stomatal defense in closing the stomata as a perception of microbe-associated molecular patterns is an effective way to inhibit the infiltration.
光是可以通过保持气孔开放和为微生物提供光合作用产物从而在叶菜类中发挥作用的因素之一。我们模拟了细菌在植物叶肉光合作用响应下在叶组织内的趋化性运输。该模型包括二氧化碳、氧气、碳酸氢盐、蔗糖/葡萄糖、细菌和叶组织内的自诱导物-2 的运输。考虑了叶绿体中碳固定和植物细胞线粒体呼吸，以及细菌群落中的运动性、趋化性、营养消耗和通讯等生物过程。我们表明，光是通过打开气孔并向叶组织内的光合作用产物输送，足以促进细菌的趋化性。细菌的趋化能力是渗透的主要因素，而植物气孔防御则通过关闭气孔来感知微生物相关分子模式，这是抑制渗透的有效方法。
