Institute of Environmental Science, and School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China.
J Mater Chem B. 2020 Jun 21;8(23):5089-5095. doi: 10.1039/d0tb00443j. Epub 2020 May 14.
Multifunctional N-doped carbon nanodots (N-CNDs) with a fluorescence (FL) quantum yield (QY) of 13.6% have been synthesized via a facile one-step hydrothermal process using Artemisia annua and 1,2-ethylenediamine as precursors. As-prepared N-CNDs showed excellent FL properties and were developed as a multifunctional sensing platform for vitamin B (VB) and Co determination, and bioimaging in living cells and zebrafish. The FL of N-CNDs is quenched efficiently in the presence of VB on the basis of the inner filter effect (IFE) or Co by static quenching, respectively. EDTA as a masking agent enables Co to be effectively eliminated and N-CNDs were used to selectively detect VB in the presence of both VB and Co. The present FL nanosensor can detect VB and Co in the linear ranges of 0.5-35 μM and 2.5-25 μM with the corresponding detection limits of 47.4 nM and 230.5 nM, respectively. The study proved that the determination of Co was based on the static quenching to form a complex between the amino group of N-CNDs and Co. Inspired by these outstanding properties, practical applications of this nanosensor for the detection of VB in actual samples (human serum, egg yolk, VB tablets and VB injection) and Co in water samples were further verified with satisfactory results. The as-constructed N-CNDs have negligible toxicity and good biocompatibility, which facilitates utilization of N-CNDs in bioimaging of A549 cells and zebrafish, and sensing VB in living cells.
通过简便的一步水热法，以黄花蒿和 1,2-乙二胺为前驱体，合成了具有荧光（FL）量子产率（QY）为 13.6%的多功能 N 掺杂碳纳米点（N-CNDs）。所制备的 N-CNDs 表现出优异的 FL 性质，并被开发为多功能传感平台，用于维生素 B（VB）和 Co 的测定以及活细胞和斑马鱼中的生物成像。VB 基于内滤效应（IFE）或 Co 通过静态猝灭有效地猝灭 N-CNDs 的 FL。EDTA 作为掩蔽剂，可有效消除 Co，并且在 VB 和 Co 存在的情况下，N-CNDs 可用于选择性检测 VB。该 FL 纳米传感器可在 0.5-35 μM 和 2.5-25 μM 的线性范围内检测 VB 和 Co，相应的检测限分别为 47.4 nM 和 230.5 nM。该研究证明 Co 的测定是基于氨基与 Co 之间形成配合物的静态猝灭。受这些优异性能的启发，进一步验证了该纳米传感器在实际样品（人血清、蛋黄、VB 片剂和 VB 注射液）中 VB 和水样中 Co 的实际检测中的实际应用，结果令人满意。构建的 N-CNDs 具有可忽略的毒性和良好的生物相容性，这有利于在 A549 细胞和斑马鱼的生物成像以及活细胞中 VB 的检测中利用 N-CNDs。
