评估秀丽隐杆线虫单一种群生育力的方法。
Methods for Assessing Fertility in C. elegans from a Single Population.
作者信息
Yen Chia-An, Curran Sean P
机构信息
Leonard Davis School of Gerontology, University of Southern California, Los Angeles, CA, USA.
Department of Molecular and Computational Biology, Dornsife College of Letters, Arts, and Sciences, University of Southern California, Los Angeles, CA, USA.
出版信息
Methods Mol Biol. 2020;2144:91-102. doi: 10.1007/978-1-0716-0592-9_8.
Reproductive senescence occurs in a wide range of species with mechanistic aspects that are conserved from Caenorhabditis elegans to humans. Genetic and environmental factors can influence fertility and reproductive output can impact rates of aging. The C. elegans Bristol N2 strain commonly used in laboratories is hermaphroditic, producing a defined number of sperm during larval development before switching exclusively to oogenesis. Here we show a method of assaying both oocyte and sperm quality from a single population of animals.
生殖衰老发生在广泛的物种中，其机制从秀丽隐杆线虫到人类都是保守的。遗传和环境因素会影响生育能力，而生殖产出会影响衰老速度。实验室中常用的秀丽隐杆线虫布里斯托N2品系是雌雄同体的，在幼虫发育期间会产生一定数量的精子，之后便 exclusively 转向卵子发生。在这里，我们展示了一种从单一动物群体中检测卵母细胞和精子质量的方法。
注
原文中“exclusively”这个词在提供的翻译内容里保留了英文，因为不清楚准确含义，无法准确翻译。你可根据实际情况替换为准确的中文表述。
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