Häkkinen L, Yli-Urpo A, Heino J, Larjava H
Institute of Dentistry, University of Turku, Finland.
J Biomed Mater Res. 1988 Nov;22(11):1043-59. doi: 10.1002/jbm.820221108.
The attachment and spreading of cultured fibroblasts on potentially bioactive glasses (bioglasses) of ten different compositions were studied. Human gingival fibroblasts were allowed to attach and spread on bio-glasses for 1-72 h. Unreactive silica glass and cell culture polystyrene served as controls. The attachment and spreading of cells were examined by 3H-thymidine labeling of cells, planimetric analysis, cytological staining, immunocytochemistry, and scanning electron microscopy. The cell attachment to bioglasses and silica glass and the cell spreading on bioglasses were slower and cell morphology more elongated compared to control plastic. In spite of great differences in bioglass compositions no great differences in cell behavior on these surfaces were detected. Thus the initial events in the tissue-implant interface might be independent on the bioglass composition, and furthermore the differences in the organization of the tissue-implant interface in vivo might depend on the nature of the surrounding tissues and subsequent changes of the implant surface and the extracellular environment.
研究了培养的成纤维细胞在十种不同成分的潜在生物活性玻璃（生物玻璃）上的附着和铺展情况。将人牙龈成纤维细胞接种在生物玻璃上，使其附着和铺展1 - 72小时。无反应性的石英玻璃和细胞培养聚苯乙烯作为对照。通过细胞的³H - 胸腺嘧啶核苷标记、平面测量分析、细胞学染色、免疫细胞化学和扫描电子显微镜检查细胞的附着和铺展情况。与对照塑料相比，细胞在生物玻璃和石英玻璃上的附着以及在生物玻璃上的铺展较慢，细胞形态更细长。尽管生物玻璃成分存在很大差异，但未检测到细胞在这些表面上行为的显著差异。因此，组织 - 植入物界面的初始事件可能与生物玻璃成分无关，此外，体内组织 - 植入物界面组织方式的差异可能取决于周围组织的性质以及植入物表面和细胞外环境的后续变化。
