通过经口胃管给予四氯化碳（CCl₄）诱导大鼠急性肝损伤。
Inducing Acute Liver Injury in Rats via Carbon Tetrachloride (CCl4) Exposure Through an Orogastric Tube.
作者信息
Frank Dmitry, Savir Shiri, Gruenbaum Benjamin F, Melamed Israel, Grinshpun Julia, Kuts Ruslan, Knyazer Boris, Zlotnik Alexander, Vinokur Max, Boyko Matthew
机构信息
Department of Anesthesiology and Critical Care, Soroka Medical Center, Ben-Gurion University of the Negev.
Department of Anesthesiology, Yale University School of Medicine.
出版信息
J Vis Exp. 2020 Apr 28(158). doi: 10.3791/60695.
Acute liver injury (ALI) plays a crucial role in the development of hepatic failure, which is characterized by severe liver dysfunction including complications such as hepatic encephalopathy and impaired protein synthesis. Appropriate animal models are vital to test the mechanism and pathophysiology of ALI and investigate different hepatoprotective strategies. Due to its ability to perform chemical transformations, carbon tetrachloride (CCl4) is widely used in the liver to induce ALI through the formation of reactive oxygen species. CCl4 exposure can be performed intraperitoneally, by inhalation, or through a nasogastric or orogastric tube. Here, we describe a rodent model, in which ALI is induced by CCl4 exposure through an orogastric tube. This method is inexpensive, easily performed, and has minimal hazard risk. The model is highly reproducible and can be widely used to determine the efficacy of potential hepatoprotective strategies and assess markers of liver injury.
急性肝损伤（ALI）在肝衰竭的发展过程中起着关键作用，肝衰竭的特征是严重的肝功能障碍，包括肝性脑病和蛋白质合成受损等并发症。合适的动物模型对于测试ALI的机制和病理生理学以及研究不同的肝保护策略至关重要。由于四氯化碳（CCl4）具有进行化学转化的能力，它在肝脏中被广泛用于通过形成活性氧来诱导ALI。CCl4暴露可以通过腹腔注射、吸入或经鼻胃管或口胃管进行。在此，我们描述一种啮齿动物模型，其中通过经口胃管暴露CCl4来诱导ALI。该方法成本低廉、易于操作且危害风险极小。该模型具有高度可重复性，可广泛用于确定潜在肝保护策略的疗效并评估肝损伤标志物。
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