化脓性链球菌毒力因子的检测

Detection of Streptococcus pyogenes Virulence Factors.

作者信息

Kozińska Aleksandra, Sitkiewicz Izabela

机构信息

Department of Drug Biotechnology and Bioinformatics, National Medicines Institute, Warszawa, Poland.

出版信息

Methods Mol Biol. 2020;2136:3-16. doi: 10.1007/978-1-0716-0467-0_1.

DOI:10.1007/978-1-0716-0467-0_1

PMID:32430809

Abstract

Streptococcus pyogenes encodes multiple virulence factors and their presence is often related to the severity of the disease. We designed the system of four low-volume multiplex PCR reactions to detect genes encoding 20 virulence factors: spd3, sdc, sdaB, sdaD, speB, spyCEP, scpA, mac, sic, speL, speK, speM, speC, speI, speA, speH, speG, speJ, smeZ, and ssa. Classification of strains based on the virulence factors absence or presence correlates with PFGE MLST and emm typing results. The typing/detection system is fast and cost-effective, can be used to detect GAS virulence factors and as a rapid tool to effectively differentiate between strains.

摘要

化脓性链球菌编码多种毒力因子，其存在通常与疾病的严重程度相关。我们设计了四个小体积多重PCR反应体系，以检测编码20种毒力因子的基因：spd3、sdc、sdaB、sdaD、speB、spyCEP、scpA、mac、sic、speL、speK、speM、speC、speI、speA、speH、speG、speJ、smeZ和ssa。基于毒力因子缺失或存在的菌株分类与PFGE MLST和emm分型结果相关。该分型/检测系统快速且具有成本效益，可用于检测A群链球菌毒力因子，并作为有效区分菌株的快速工具。