Pablo Abed H, Breaud Autumn R, Clarke William
Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland.
Curr Protoc Toxicol. 2020 Jun;84(1):e92. doi: 10.1002/cptx.92.
Immunosuppressant medications help suppress the immune system response through inhibition of various checkpoints in the regulatory biochemical pathway. This is useful in prevention of organ rejection in transplantation or in the treatment of autoimmune diseases such as lupus or rheumatoid arthritis. Quantification of immunosuppressive drugs in blood is needed clinically for optimization of treatment and to avoid toxicity or unwanted side effects. Here, we describe a quantitative method to determine the concentration of cyclosprine A, tacrolimus, sirolimus, and everolimus in whole blood. This method has been used for many years clinically to support patient care. © 2020 by John Wiley & Sons, Inc.
免疫抑制药物通过抑制调节生化途径中的各种检查点来帮助抑制免疫系统反应。这在预防移植中的器官排斥或治疗自身免疫性疾病（如狼疮或类风湿性关节炎）方面很有用。临床上需要对血液中的免疫抑制药物进行定量，以优化治疗并避免毒性或不良副作用。在此，我们描述了一种测定全血中环孢素A、他克莫司、西罗莫司和依维莫司浓度的定量方法。该方法已在临床上使用多年以支持患者护理。© 2020约翰威立父子公司版权所有
