Department of Microbiology and Molecular Genetics, University of the Punjab, Lahore, 54590, Pakistan.
Nur International University, Lahore, Pakistan.
Arch Microbiol. 2020 Sep;202(7):1907-1913. doi: 10.1007/s00203-020-01912-0. Epub 2020 May 24.
In this work, volatile fatty acids (VFAs) were used as a carbon source to assess the ability of bacteria present in waste activated sludge (WAS), as indigenous flora, to accumulate polyhydroxyalkanoates (PHA). Acetic acid and propionic acid were used both separately and in combination as feedstock, producing either homopolymer poly(3-hydroxybutyrate) (3PHB) and/or the co-polymer, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) P(3HB-co-3HV). The overall potential to use waste activated sludge as biomass for production of valuable polymers was assessed, and a quality assessment of the as-produced polymers was run, with the extracted polymer being analyzed for properties such as thermal, microstructure and molecular weight. It was found that a blend of copolymers was typically produced, with thermal properties being similar to those reported elsewhere. The overall PHA cell content obtained was 0.29 gPHA gVSS.
在这项工作中，挥发性脂肪酸（VFAs）被用作碳源，以评估存在于废活性污泥（WAS）中的细菌（作为土著菌群）积累聚羟基烷酸酯（PHA）的能力。分别使用乙酸和丙酸以及它们的混合物作为原料，生产均聚物聚（3-羟基丁酸酯）（3PHB）和/或共聚物聚（3-羟基丁酸酯-3-羟基戊酸酯）P（3HB-co-3HV）。评估了将废活性污泥作为生产有价值聚合物的生物质的总体潜力，并对所生产的聚合物进行了质量评估，对提取的聚合物进行了热性能、微观结构和分子量等特性的分析。结果发现，通常会生产出共聚物的混合物，其热性能与其他地方报道的相似。获得的总 PHA 细胞含量为 0.29 gPHA gVSS。
