Zhang Yunlin, Gao Mengyu, Hu Meijuan, Zhang Bingqi, He Yuting, Li Shun, Bao Ji, Bu Hong
Laboratory of Pathology, Key Laboratory of Transplant Engineering and Immunology, NHFPC, West China Hospital, Sichuan University, Chengdu, China.
Laboratory of Pathology, Key Laboratory of Transplant Engineering and Immunology, NHFPC, West China Hospital, Sichuan University, Chengdu, China; Department of Pathology, West China Hospital, Sichuan University, Chengdu, China.
Transplant Proc. 2020 Jul-Aug;52(6):1906-1909. doi: 10.1016/j.transproceed.2020.02.138. Epub 2020 May 24.
Genetically modified pigs are the first choice for xenotransplantation research, but there have been problems with monoclonal screening of edited cells before nuclear transfer. Our objective was to get a novel strategy to quickly obtain monoclonal cells with low damage by microarray and to produce efficient gene-editing monoclonal cells in batches. Micropattern array printing technology was introduced to limit only a single cell was adhered on a micropattern substrate, and after 4 days of culture, the single cell grew into a monoclonal cell sphere and then came off from the bottom of the petri dish automatically. After sequencing, the results showed that a single cell is confined to a micropattern and grows into a sphere of monoclonal cells.
基因编辑猪是异种移植研究的首选，但在核移植前对编辑细胞进行单克隆筛选存在问题。我们的目标是获得一种新策略，通过微阵列快速获得低损伤的单克隆细胞，并批量生产高效的基因编辑单克隆细胞。引入微图案阵列打印技术，使微图案基质上仅附着单个细胞，培养4天后，单个细胞生长成单克隆细胞球，然后自动从培养皿底部脱落。测序结果表明，单个细胞被限制在微图案中并生长成单克隆细胞球。
