具有益生菌潜力的土著酵母对发酵的 Guajillo 辣椒调味汁香气特征的贡献。
Contribution of autochthonous yeasts with probiotic potential to the aroma profile of fermented Guajillo pepper sauce.
机构信息
Instituto de Agroquímica y Tecnología de Alimentos (IATA-CSIC) Avda, Agustín Escardino 7, 46980 Paterna, Valencia, Spain.
Instituto Politécnico Nacional, Escuela Nacional de Ciencias Biológicas. Unidad Profesional Adolfo López Mateos. Av. Wilfrido Massieu S/N esq. Manuel L. Stampa, Nueva Industrial Vallejo, Gustavo A. Madero, C.P. 07738, Ciudad de México, Mexico.
出版信息
J Sci Food Agric. 2020 Oct;100(13):4940-4949. doi: 10.1002/jsfa.10556. Epub 2020 Jul 1.
BACKGROUND
Three yeast strains with probiotic potential, Hanseniaspora opuntiae, Pichia kudriavzevii, and Wickerhamomyces anomalus were inoculated in the fermentation of Guajillo chilli pepper (Capsicum annuum L.) sauce, and the different aroma profiles were investigated. Using headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) analysis and gas chromatography-olfactometry (GCO), flavour compound production was evaluated during the fermentation of the Guajillo chilli pepper sauces.
RESULTS
A total of 78 volatile compounds were identified during the yeast fermentation of the sauce. Most aldehydes and terpenes detected were present at the beginning of the fermentation, indicating a Guajillo chilli pepper origin. Among the 34 active aroma compounds detected by GCO, propanoic acid (cheesy), 3-methylbutanoic acid (sharp, cheese), ethyl 2-methylbutanoate (fruity), and 6-methyl-5-hepten-2-one (strong, citrus) were identified as key aroma contributors produced by the inoculation of the yeasts. A different aroma profile was produced by probiotic yeast. Hanseniaspora opuntiae produced an aroma profile with herbal and green notes based on high production of aldehydes, ketones, and acetic acid. Pichia kudriavzevii and W. anomalus produced fruity, green-herbal, and cheesy notes based on ester compounds, alcohol and branched-chain acids production although, the production of propanoic acid by W. anomalus increased the cheesy character in the sauces.
CONCLUSION
The aroma profile of fermented chilli pepper sauces depends not only on the chili pepper varieties used but also on the fermentation process as a source of aroma compounds. The use of probiotic yeast can be used to improve and diversify the aroma profile of fermented chilli pepper sauces. © 2020 Society of Chemical Industry.
背景
将具有益生菌潜力的三种酵母菌株——汉逊德巴利酵母、库德里阿兹威毕赤酵母和异常威克汉姆酵母接种到墨西哥青辣椒酱的发酵过程中,并研究了不同的香气特征。使用顶空固相微萃取-气相色谱-质谱联用(HS-SPME-GC-MS)分析和气相色谱-嗅觉测量(GCO)评估了辣椒酱发酵过程中风味化合物的产生。
结果
在辣椒酱的酵母发酵过程中共鉴定出 78 种挥发性化合物。在发酵初期检测到的大多数醛类和萜烯类化合物都来自于墨西哥青辣椒。在通过 GCO 检测到的 34 种活性香气化合物中,丙酸(奶酪味)、3-甲基丁酸(尖锐,奶酪味)、乙基 2-甲基丁酸酯(果香)和 6-甲基-5-庚烯-2-酮(强烈,柑橘味)被鉴定为接种酵母产生的关键香气贡献物。益生菌酵母产生了不同的香气特征。汉逊德巴利酵母产生的香气特征具有草药和绿色调,这是基于醛类、酮类和乙酸的高产量。库德里阿兹威毕赤酵母和异常威克汉姆酵母产生果香、绿色草药和奶酪味,这是基于酯类化合物、醇类和支链酸的产生,尽管异常威克汉姆酵母产生的丙酸增加了辣椒酱的奶酪味。
结论
发酵辣椒酱的香气特征不仅取决于所使用的辣椒品种,还取决于作为香气化合物来源的发酵过程。使用益生菌酵母可以改善和多样化发酵辣椒酱的香气特征。