Soluble epoxide hydrolase inhibitors from (Wall.) Decne.

Nine bioactive compounds, including one new dihydroisocoumarin glycoside, 3-thunberginol C 6---D-glucopyranoside (), were isolated by chromatographic separation from the fruits of the Vietnamese medicinal plant (Wall.) Decne. 3-thunberginol C 6---D-glucopyranoside was determined as a mixture of boat-like conformers based on NMR evidence and density functional theory (DFT) calculations. The inhibition of soluble epoxide hydrolase (sEH) by the isolated compounds was comparable to that of AUDA (positive control), yielding IC values ranging from 10.0 ± 0.6 to 88.4 ± 0.2 µM. Among isolated compounds, 3-methoxy-4-hydroxy-benzoic acid () and 2',6'-dihydroxy 3',4'-dimethoxychalcone () were identified as a potent inhibitor of sEH, with IC values of 19.3 ± 2.2 and 10.0 ± 0.6 mM, respectively. These results suggest that the fruits of may be useful as daily supplements for the prevention of cardiovascular and other sEH-related diseases.[Figure: see text].