应用磁珠从猪带绦虫免疫血清中提纯 IgM 抗体
Use of magnetic particles in the purification of IgM antibodies against Taenia solium.
机构信息
Laboratorio de Inmunología Parasitaria, Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, Lima, Perú.
Unidad de Cisticercosis, Instituto Nacional de Ciencias Neurológicas, Lima, Perú.
出版信息
Rev Peru Med Exp Salud Publica. 2020 Jan-Mar;37(1):104-109. doi: 10.17843/rpmesp.2020.371.4628. Epub 2020 Jun 8.
The use of L protein coupled magnetic particles for the concentration and purification of immunoglobulin M (mIgM) monoclonal antibodies against Taenia solium was evaluated. Three concentration methods and different elution times were evaluated and the ratio of particles to the ratio of mIgM was optimized. It is demonstrated that: 1) with the use of magnetic particles, a previous concentration of mIgM is not required, which reduces the manipulation of the antibodies and improves the recovery, 2) the use of a binding buffer can be omitted, since the pH of most cell culture supernatants are neutral, and 3) longer elution times (~ 45 minutes) are needed to increase recovery to a level greater than 80%. The study demonstrates that the use of L protein-coupled magnetic particles is a simple and efficient tool for mIgM concentration and purification.
使用 L 蛋白偶联磁珠来浓缩和纯化针对猪带绦虫的免疫球蛋白 M（mIgM）单克隆抗体进行了评估。评估了三种浓缩方法和不同的洗脱时间，并优化了颗粒与 mIgM 的比例。结果表明：1）使用磁珠不需要预先浓缩 mIgM，减少了抗体的操作，提高了回收率；2）可以省略结合缓冲液的使用，因为大多数细胞培养上清液的 pH 值为中性；3）需要更长的洗脱时间（~45 分钟）才能将回收率提高到 80%以上。该研究表明，使用 L 蛋白偶联磁珠是浓缩和纯化 mIgM 的一种简单有效的工具。
Zotero 插件