支持基于 TMV 病毒载体的 Q-Griffithsin 符合 cGMP 生产的质粒和病毒库的稳定性。
Stability of plasmid and viral banks supporting the cGMP manufacture of Q-Griffithsin from a TMV-based viral vector.
机构信息
Center for Predictive Medicine, University of Louisville School of Medicine, Louisville KY, USA.
James Graham Brown Cancer Center, University of Louisville School of Medicine,Louisville KY, USA; Center for Predictive Medicine, University of Louisville School of Medicine, Louisville KY, USA; Department of Medicine, University of Louisville School of Medicine, Louisville KY, USA.
出版信息
J Biotechnol. 2020 Aug 20;320:74-76. doi: 10.1016/j.jbiotec.2020.06.009. Epub 2020 Jun 15.
Abstract
The "whole genome" TMV-based expression system, Geneware®, was used in the cGMP production of the plant-made pharmaceutical Q-Griffithsin and demonstrates stable expression for up to a two-year period. Virion and plasmid banks which contained viral cDNA and a Q-Griffithsin sequence were able to produce >200 g of Q-Griffithsin. Data assessing the quality and stability of the product banks were measured through functional assessments of visual symptomology and product expression.
摘要
该“全基因组”TMV 表达系统，Geneware®，用于植物源药物 Q-Griffithsin 的 cGMP 生产，其展示了长达两年的稳定表达。包含病毒 cDNA 和 Q-Griffithsin 序列的病毒粒子和质粒库能够生产超过 200g 的 Q-Griffithsin。通过对视觉症状和产物表达的功能评估来衡量对产物库的质量和稳定性数据。
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