Dipartimento di Medicina Sperimentale e Clinica, Sezione Scienze Fisiologiche, Università degli Studi di Firenze, Viale G.B. Morgagni 63, 50134, Firenze, Italy.
Dipartimento di Medicina Sperimentale e Clinica, Sezione Scienze Fisiologiche, Università degli Studi di Firenze, Viale G.B. Morgagni 63, 50134, Firenze, Italy.
Respir Physiol Neurobiol. 2020 Sep;280:103482. doi: 10.1016/j.resp.2020.103482. Epub 2020 Jun 15.
The role of the different components of the respiratory network in the mediation of opioid-induced respiratory depression is still unclear. We investigated the contribution of the preBötzinger Complex (preBötC) and the neighbouring Bötzinger Complex (BötC) and inspiratory portion of the ventral respiratory group (iVRG) in anesthetized, vagotomized, paralyzed and artificially ventilated adult rabbits making use of bilateral microinjections (30-50 nl) of the μ-opioid receptor agonist [D-Ala, N-Me-Phe, Gly-ol]-enkephalin (DAMGO). Dose-dependent effects were observed. In the preBötC and the BötC 0.1 mM DAMGO microinjections caused mainly reductions in peak phrenic amplitude associated with tonic phrenic activity and irregular (ataxic) patterns of breathing that were more pronounced in the preBötC. Apneic effects developed at 0.5 mM. In the iVRG DAMGO provoked decreases in amplitude and frequency of phrenic bursts at 0.1 mM and apnea at 0.5 mM. Local 5 mM naloxone reversed the apneic effects. The results imply that different components of the respiratory network may contribute to opioid-induced respiratory disorders.
不同呼吸网络成分在介导阿片类药物引起的呼吸抑制中的作用尚不清楚。我们利用双侧微量注射（30-50nl）μ-阿片受体激动剂[D-Ala，N-Me-Phe，Gly-ol]-脑啡肽（DAMGO），在麻醉、迷走神经切断、麻痹和人工通气的成年兔中研究了 PreBötzinger 复合体（preBötC）和邻近的 Bötzinger 复合体（BötC）以及腹侧呼吸组吸气部分（iVRG）的贡献。观察到剂量依赖性效应。在 preBötC 和 BötC 中，0.1mM DAMGO 微注射主要引起峰膈肌幅度的减少，伴有膈肌紧张活动和不规则（共济失调）的呼吸模式，在 preBötC 中更为明显。0.5mM 时出现呼吸暂停效应。在 iVRG 中，DAMGO 在 0.1mM 时引起膈神经爆发幅度和频率的降低，在 0.5mM 时引起呼吸暂停。局部 5mM 纳洛酮逆转了呼吸暂停效应。结果表明，呼吸网络的不同成分可能有助于阿片类药物引起的呼吸障碍。
