Shanghai Collaborative Innovation Centre for Aquatic Animal Genetics and Breeding, Shanghai Ocean University, Shanghai, 201306, China.
Centre for Research on Environmental Ecology and Fish Nutrition of the Ministry of Agriculture, Shanghai Ocean University, Shanghai, 201306, China.
Cell Tissue Res. 2020 Nov;382(2):367-379. doi: 10.1007/s00441-020-03223-8. Epub 2020 Jun 15.
The ridgetail white prawn Exopalaemon carinicauda has the potential to be used as a model organism in crustacean research because it has a transparent body, available draft genome, and short life cycle. However, their ovarian development pattern remains unclear under laboratory culture conditions. This study investigated the changes of ovarian external feature, ovarian histology, gonadosomatic index (GSI), and hepatosomatic index (HSI), as well as the expression and localization of vitellogenin in the ovary and the hepatopancreas during the first ovarian development cycle of E. carinicauda under laboratory-reared condition. The results demonstrated that (1) the first ovarian development cycle of E. carinicauda could be divided into 5 different stages in which the ovary changes its color from white to yellow during the vitellogenesis process in parallel with increasing GSI. (2) After pubertal molt, most females reached ovarian stage II while the females reached stage V after premating molt. (3) During the ovarian development, GSI increased smoothly and HSI relatively stable during the period of stages I to IV, while GSI increased but HSI decreased significantly from stages IV to V. (4) In situ hybridization (ISH) revealed that EcVg was slightly expressed in the oocyte cytoplasm of previtellogenic oocytes. The positive signal was mainly detected in hepatopancreatic fibrillar cells, and a strong signal was found in the hepatopancreas at stage IV. Moreover, the expression level of EcVg-mRNA in the hepatopancreas is stage-specific, and the hepatopancreas contributes majority of vitellin precursor protein to support the ovarian development of E. carinicauda.
脊尾白虾（Exopalaemon carinicauda）具有成为甲壳动物研究模型生物的潜力，因为它具有透明的身体、可用的基因组草图和较短的生命周期。然而，在实验室培养条件下，其卵巢发育模式仍不清楚。本研究调查了脊尾白虾在实验室养殖条件下第一次卵巢发育周期中卵巢外部特征、组织学、性腺指数（GSI）和肝体指数（HSI）的变化，以及卵黄原蛋白（Vg）在卵巢和肝胰腺中的表达和定位。结果表明：（1）脊尾白虾的第一次卵巢发育周期可分为 5 个不同阶段，在此过程中，卵巢在卵黄发生过程中从白色变为黄色，同时 GSI 增加。（2）在青春期蜕皮后，大多数雌性达到卵巢阶段 II，而在交配前蜕皮后，雌性达到阶段 V。（3）在卵巢发育过程中，GSI 平稳增加，HSI 在 I 至 IV 阶段相对稳定，而 GSI 从 IV 阶段增加，但 HSI 从 IV 阶段到 V 阶段显著降低。（4）原位杂交（ISH）显示 EcVg 在卵黄发生前卵母细胞的卵母细胞质中略有表达。阳性信号主要在肝胰腺纤维细胞中检测到，在第 IV 阶段在肝胰腺中发现强信号。此外，EcVg-mRNA 在肝胰腺中的表达水平具有阶段特异性，肝胰腺为脊尾白虾的卵巢发育提供了大部分卵黄前体蛋白。
