Yukphan Pattaraporn, Charoenyingcharoen Piyanat, Malimas Sukunphat, Muramatsu Yuki, Nakagawa Yasuyoshi, Tanasupawat Somboon, Yamada Yuzo
Microbial Diversity and Utilization Research Team, Thailand Bioresource Research Center (TBRC), National Center for Genetic Engineering and Biotechnology (BIOTEC), Pathumthani 12120, Thailand.
Rungrueng-Fertilizer.co, Ltd. 207/5 Moo1, Nong Ya, Muang Kanchanaburi, Kanchanaburi 71000, Thailand.
Int J Syst Evol Microbiol. 2020 Jul;70(7):4351-4357. doi: 10.1099/ijsem.0.004292.
Two bacterial strains, isolates AC10 and AC20, which were reported in a previous study on the diversity of acetic acid bacteria in Thailand, were subjected to a taxonomic study. The phylogenetic analysis based on the 16S rRNA gene sequences showed that the two isolates were located closely to the type strains of and . However, the two isolates formed a separate cluster from the type strains of the two species. The genomic DNA of isolate AC10 was sequenced. The assembled genomes of the isolate were analysed for average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH). The results showed that the highest ANI and dDDH values between isolate AC10 and DSM 3503 were 91.15 and 68.2 %, which are lower than the suggested values for species delineation. The genome-based tree was reconstructed and the phylogenetic lineage based on genome sequences showed that the lineage of isolate AC10 was distinct from DSM 3503 and its related species. The two isolates were distinguished from and their relatives by their phenotypic characteristics and MALDI-TOF profiles. Therefore, the two isolates, AC10 (=BCC 15749=TBRC 11329=NBRC 103576) and AC20 (=BCC 15759=TBRC 11330=NBRC 103579), can be assigned to an independent species within the genus , and the name sp. nov. is proposed for the two isolates.
在之前一项关于泰国醋酸菌多样性的研究中报道的两株细菌菌株AC10和AC20，接受了分类学研究。基于16S rRNA基因序列的系统发育分析表明，这两株分离株与[具体菌种1]和[具体菌种2]的模式菌株位置相近。然而，这两株分离株与这两个物种的模式菌株形成了一个单独的聚类。对分离株AC10的基因组DNA进行了测序。对该分离株的组装基因组进行了平均核苷酸同一性（ANI）和数字DNA-DNA杂交（dDDH）分析。结果表明，分离株AC10与[具体菌种1]DSM 3503之间的最高ANI和dDDH值分别为91.15%和68.2%，低于物种划分的建议值。重建了基于基因组的树，基于基因组序列的系统发育谱系表明，分离株AC10的谱系与[具体菌种1]DSM 3503及其相关物种不同。这两株分离株通过其表型特征和基质辅助激光解吸电离飞行时间质谱（MALDI-TOF）图谱与[具体菌种1]及其亲缘种区分开来。因此，这两株分离株AC10（=BCC 15749=TBRC 11329=NBRC 103576）和AC20（=BCC 15759=TBRC 11330=NBRC 103579）可归为[具体属名]属内的一个独立物种，并为这两株分离株提出了新物种名称[新物种名称]。
