转化生长因子α（TGFα）诱导C3H 10T1/2细胞中C-FOS和C-MYC的表达。

Transforming growth factor alpha (TGF alpha) induction of C-FOS and C-MYC expression in C3H 10T1/2 cells.

作者信息

Cutry A F, Kinniburgh A J, Twardzik D R, Wenner C E

机构信息

Department of Experimental Biology, Roswell Park Memorial Institute, Buffalo, NY 14263.

出版信息

Biochem Biophys Res Commun. 1988 Apr 15;152(1):216-22. doi: 10.1016/s0006-291x(88)80702-2.

DOI:10.1016/s0006-291x(88)80702-2

PMID:3258745

Abstract

We have investigated the effects of transforming growth factor alpha (TGF alpha) in C3H10T1/2 cells, on S phase entry and early gene activation events associated with cell cycle progression. We find that EGF and TGF alpha, which both utilize the EGF receptor for signal generation, are able to stimulate DNA synthesis in these cells with nearly superimposable kinetics; however, the stimulation by TGF alpha was slightly greater at nearly all time points assayed. This report is the first showing that TGF alpha, like EGF, vigorously induces c-myc and c-fos gene expression in these cells. A significant stimulation of c-myc and c-fos mRNA levels is observed with both TGF alpha and EGF; c-myc mRNA levels show an 8-fold induction with both mitogens, while c-fos inductions were on the order of 12 to 14-fold at maximum. However, the induction of c-myc mRNA by TGF alpha has slower kinetics than by EGF.

摘要

我们研究了转化生长因子α（TGFα）对C3H10T1/2细胞进入S期以及与细胞周期进程相关的早期基因激活事件的影响。我们发现，表皮生长因子（EGF）和TGFα均利用EGF受体来产生信号，二者都能够以几乎重叠的动力学刺激这些细胞中的DNA合成；然而，在几乎所有检测的时间点，TGFα的刺激作用都略强一些。本报告首次表明，TGFα与EGF一样，能在这些细胞中强烈诱导c-myc和c-fos基因表达。TGFα和EGF均能显著刺激c-myc和c-fos mRNA水平；两种有丝分裂原均使c-myc mRNA水平诱导增加8倍，而c-fos的诱导倍数最大可达12至14倍。然而，TGFα诱导c-myc mRNA的动力学比EGF慢。