液相色谱-质谱法分析和转化寡核苷酸治疗药物。
BIOANALYSIS AND BIOTRANSFORMATION OF OLIGONUCLEOTIDE THERAPEUTICS BY LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY.
机构信息
Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy, University of Georgia, 250 West Green Street, Athens, GA, 30602-2352.
Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Ain Shams University, Organization of African Unity Street, Cairo, 11566, Egypt.
出版信息
Mass Spectrom Rev. 2021 Jul;40(4):334-358. doi: 10.1002/mas.21641. Epub 2020 Jun 26.
Since 2016, eight new oligonucleotide therapies have been approved which has led to increased interest in oligonucleotide analysis. There is a particular need for powerful bioanalytical tools to study the metabolism and biotransformation of these molecules. This review provides the background on the biological basis of these molecules as currently used in therapies. The article also reviews the current state of analytical methodology including state of the art sample preparation techniques, liquid chromatography-mass spectrometry methods, and the current limits of detection/quantitation. Finally, the article summarizes the challenges in oligonucleotide bioanalysis and provides future perspectives for this emerging field. © 2020 John Wiley & Sons Ltd.
自 2016 年以来，已有 8 种新型寡核苷酸疗法获得批准，这使得人们对寡核苷酸分析产生了浓厚的兴趣。特别需要强大的生物分析工具来研究这些分子的代谢和生物转化。本文综述了目前用于治疗的这些分子的生物学基础。文章还回顾了分析方法的现状，包括最新的样品制备技术、液相色谱-质谱法以及当前的检测/定量下限。最后，文章总结了寡核苷酸生物分析中的挑战，并为这一新兴领域提供了未来展望。© 2020 约翰威立父子公司
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