College of Marine Science, South China Agricultural University, Guangzhou, Guangdong, 510642, PR China; Guangdong Laboratory for Lingnan Modern Agriculture, South China Agricultural University, Guangzhou, 510642, China; Joint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation, South China Agricultural University, Guangzhou, 510642, PR China.
Key Laboratory for Heavy Metal Pollution Control and Reutilization, School of Environment and Energy, Peking University Shenzhen Graduate School, Shenzhen, 518055, China.
Fish Shellfish Immunol. 2020 Oct;105:24-34. doi: 10.1016/j.fsi.2020.06.041. Epub 2020 Jun 25.
Benzo[a]pyrene (BaP) is one of the most well studied carcinogenic polycyclic aromatic hydrocarbons (PAHs) that has been associated with a wide range of toxic effects in aquatic organisms. In the present study, the mosquitofish and zebrafish were exposed to BaP (100 μg/L) for 15 days. We analyzed the intestinal microbial community of mosquitofish and zebrafish using 16S rRNA gene amplicon sequencing and also performed transcriptional profiling of the inflammation pathway related genes in the intestinal tissues. Our results showed that BaP exposure induced similar changes to the composition of microbial community in mosquitofish and zebrafish. At the phylum level, the abundance of Proteobacteria decreased while the abundance of Firmicutes increased following BaP exposure. At the genus level, a common pathogenic genus staphylococcus significantly increased in the BaP treatment groups, compared to the control (DMSO, ~0.001% v/v). In addition, it was observed that BaP significantly increased the mRNA level of il1β in both mosquitofish and zebrafish. The transcript levels of il6, il8, il10 and ifnphi1 were significantly increased in zebrafish, however not in mosquitofish, following Bap exposure. Our findings suggest that BaP could induce microbiota dysbiosis and inflammation in the intestine of mosquitofish and zebrafish.
苯并[a]芘（BaP）是研究最广泛的致癌多环芳烃（PAH）之一，与水生生物的多种毒性作用有关。在本研究中，将食蚊鱼和斑马鱼暴露于 BaP（100μg/L）中 15 天。我们使用 16S rRNA 基因扩增子测序分析了食蚊鱼和斑马鱼的肠道微生物群落，并对肠道组织中炎症途径相关基因进行了转录谱分析。结果表明，BaP 暴露诱导了食蚊鱼和斑马鱼肠道微生物群落组成的相似变化。在门水平上，厚壁菌门的丰度下降，而拟杆菌门的丰度增加。在属水平上，与对照组（DMSO，~0.001%v/v）相比，常见的致病性葡萄球菌属在 BaP 处理组中的丰度显著增加。此外，观察到 BaP 显著增加了食蚊鱼和斑马鱼中 il1β的 mRNA 水平。在 BaP 暴露后，il6、il8、il10 和 ifnphi1 的转录水平在斑马鱼中显著增加，但在食蚊鱼中没有增加。我们的研究结果表明，BaP 可诱导食蚊鱼和斑马鱼肠道微生物失调和炎症。
