Yang Ying, Li Shuzhen, Fan Shuling, Yang Jing, Li Zheng, Zhang Henglin, Qu Yuanyuan
Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, Liaoning, China.
Sheng Wu Gong Cheng Xue Bao. 2020 Jun 25;36(6):1162-1169. doi: 10.13345/j.cjb.190426.
In recent years, selenium nanoparticles (SeNPs) have been widely used in many fields such as nanotechnology, biomedicine and environmental remediation due to their good electrical conductivity, photothermal properties and anticancer properties. In this study, the cell-free supernatant, whole cell and the cell-free extracts of the strain Cupriavidus sp. SHE were used to synthesize SeNPs, and several methods were applied to analyze the crystal structure and surface functional groups of the nanoparticles. Finally, Pseudomonas sp. PI1 (G⁺) and Escherichia coli BL21 (G⁻) were selected to investigate the antibacterial properties of SeNPs. Cell-free supernatant, whole cell and cell-free extracts of the strain could synthesize SeNPs. As for the cell-free supernatant, selenite concentration of 5 mmol/L and pH=7 were favorable for the synthesis of SeNPs. TEM images show that the average size of nanospheres synthesized by the supernatant was 196 nm. XRD analysis indicates the hexagonal crystals structure of SeNPs. FTIR and SDS-PAGE confirmed the proteins bound to the surfaces of SeNPs. SeNPs synthesized by cell-free supernatant showed no antimicrobial activities against Pseudomonas sp. PI1 and Escherichia coli BL21 (DE3). These results suggest that proteins played an important role in biotransformation of SeNPs in an eco-friendly process, and SeNPs synthesized in this study were non-toxic and biologically compatible, which might be applied in other fields in the future.
近年来，硒纳米颗粒（SeNPs）因其良好的导电性、光热性能和抗癌性能而被广泛应用于纳米技术、生物医学和环境修复等许多领域。在本研究中，利用贪铜菌属菌株SHE的无细胞上清液、全细胞和无细胞提取物合成了SeNPs，并应用多种方法分析了纳米颗粒的晶体结构和表面官能团。最后，选择了假单胞菌属菌株PI1（革兰氏阳性菌）和大肠杆菌BL21（革兰氏阴性菌）来研究SeNPs 的抗菌性能。该菌株的无细胞上清液、全细胞和无细胞提取物均可合成SeNPs。对于无细胞上清液，5 mmol/L的亚硒酸盐浓度和pH=7有利于SeNPs的合成。透射电子显微镜（TEM）图像显示，由上清液合成的纳米球平均尺寸为196 nm。X射线衍射（XRD）分析表明SeNPs具有六方晶体结构。傅里叶变换红外光谱（FTIR）和十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）证实了与SeNPs表面结合的蛋白质。由无细胞上清液合成的SeNPs对假单胞菌属菌株PI1和大肠杆菌BL21（DE3）没有抗菌活性。这些结果表明，蛋白质在SeNPs的生物转化过程中发挥了重要作用，且该过程对环境友好，本研究中合成的SeNPs无毒且具有生物相容性，未来可能应用于其他领域。
