Wu Dai-Ling, Zou Hai-Yan, He Lu-Xi, Gao Fang-Zhou, Ying Guang-Guo, He Liang-Ying
Environmental Research Institute, Guangdong Provincial Key Laboratory of Chemical Pollution and Environmental Safety, Ministry of Education Key Laboratory of Theoretical Chemistry of Environment, South China Normal University, Guangzhou 510006, China.
School of Environment, South China Normal University, Guangzhou 510006, China.
Huan Jing Ke Xue. 2020 Jul 8;41(7):3222-3230. doi: 10.13227/j.hjkx.201911256.
Intensive use of antibiotics promotes the occurrence and development of antibiotic resistance. Antibiotic resistance genes (ARGs) enter water environments from human and animal sources. Groundwater serves as an important water supply, while the profiles and risk of ARGs in groundwater remain unknown. The abundance and profiles of ARGs in 11 domestic wells in the Maozhou River basin of Shenzhen City were analyzed by high-throughput qPCR. The results showed that a total of 141 ARGs and 8 mobile genetic elements (MGEs) were detected, of which the genes corresponding resistance to sulfonamides, multidrugs, and aminoglycosides were the most abundant. In each well, the number of detected ARGs and MGEs ranged from 48 to 89, with an average of 68. When normalized by the abundance of 16S rRNA genes, it was found that each bacterium carried at least one ARG in the groundwater of W7, W8, and W10, while in W11, each bacterium carried at least four ARGs. Clinically relevant ARGs that code for resistance to glycopeptide (, , , and ), -lactams ( and ), or chloramphenicol () were found in groundwater. In addition, the abundance of sulfonamides, multidrugs, aminoglycosides, -lactam, and chloramphenicol resistance genes were positively correlated with the abundance of MGEs (<0.01), suggesting that MGEs may promote the spread of ARGs in groundwater.
抗生素的大量使用促进了抗生素耐药性的产生和发展。抗生素耐药基因（ARGs）从人类和动物源进入水环境。地下水是重要的供水水源，然而地下水中ARGs的分布情况和风险仍不明确。通过高通量定量PCR分析了深圳市茅洲河流域11口水井中ARGs的丰度和分布情况。结果表明，共检测到141种ARGs和8种可移动遗传元件（MGEs），其中对磺胺类、多药和氨基糖苷类耐药的基因最为丰富。每口井中检测到的ARGs和MGEs数量在48至89个之间，平均为68个。以16S rRNA基因丰度进行标准化后发现，在W7、W8和W10的地下水中，每个细菌至少携带一个ARGs，而在W11中，每个细菌至少携带四个ARGs。在地下水中发现了对糖肽（、、、和）、β-内酰胺（和）或氯霉素（）具有耐药性的临床相关ARGs。此外，磺胺类、多药、氨基糖苷类、β-内酰胺和氯霉素耐药基因的丰度与MGEs的丰度呈正相关（<0.01），表明MGEs可能促进ARGs在地下水中的传播。
