Núcleo de Pesquisa e Conservação de Cervídeos (NUPECCE), Departamento de Zootecnia, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, Jaboticabal, Brazil.
Departamento de Zootecnia, Centro de Ciências Agrárias-Campus II, Universidade Federal da Paraíba, Areia, Brazil.
Biopreserv Biobank. 2020 Oct;18(5):351-357. doi: 10.1089/bio.2020.0012. Epub 2020 Jun 30.
Maintaining genetic variability is an important part of the conservation of endangered species, so the construction of germplasm banks is essential. Several species of the genus endure constant pressure in their natural habitat and are threatened with extinction. The correct manipulation and adequacy of the diluents and cryoprotectants must be studied to be successful in the formation of these banks. The purpose of this study was to evaluate the efficiency of three different cryoprotectants in sperm cryopreservation in the species : 6% glycerol (GLY), 3% ethylene glycol (ETG), and 5% dimethylformamide (DMF). Semen was obtained with the lateral deviation of the penis to an artificial vagina. In the pre-freeze and post-thaw periods, motility, vigor, membrane integrity, acrosome integrity, and sperm cell morphology were evaluated for each of the cryoprotectants. Post-thaw motility was higher when semen was frozen with cryoprotectants GLY and DMF (55.31 ± 7.39 and 55.94 ± 2.77, respectively), compared with the result obtained for ETG (48.13 ± 2.39). For major defects (MaD), a difference was observed between the pre- and post-cryopreservation periods, such that DMF generated a higher number of post-thaw MaD (25.94 ± 5.37). All cryoprotectants were efficient for cryopreservation of semen, resulting in samples with satisfactory viability after thawing. However, the medium with the cryoprotectants GLY, at a concentration of 6%, and DMF, at a concentration of 5%, were preferable.
维持遗传变异性是保护濒危物种的重要组成部分，因此建立种质库至关重要。属中的几个物种在其自然栖息地中持续受到压力，面临灭绝的威胁。为了成功建立这些种质库，必须研究正确的操作方法和稀释剂和冷冻保护剂的充足性。本研究旨在评估三种不同冷冻保护剂在物种精子冷冻保存中的效率：6%甘油（GLY）、3%乙二醇（ETG）和 5%二甲基甲酰胺（DMF）。通过阴茎侧向偏离人工阴道获得精液。在预冻和解冻期间，对每种冷冻保护剂的运动性、活力、膜完整性、顶体完整性和精子细胞形态进行评估。用冷冻保护剂 GLY 和 DMF 冷冻的精液解冻后运动性更高（分别为 55.31±7.39 和 55.94±2.77），而 ETG 的结果为 48.13±2.39。对于主要缺陷（MaD），在预冻和冻存期间观察到差异，即 DMF 在解冻后产生更高数量的 MaD（25.94±5.37）。所有冷冻保护剂都能有效地冷冻保存精液，解冻后样品具有令人满意的活力。然而，6%浓度的 GLY 和 5%浓度的 DMF 等冷冻保护剂介质是优选的。
