Bosnes V, Hirschberg H
Institute of Transplantation Immunology, Oslo, Norway.
J Neurooncol. 1988;6(1):85-92. doi: 10.1007/BF00163545.
We have cultured peripheral blood lymphocytes (PBL) from glioblastoma patients in recombinant interleukin-2 (IL-2) containing medium for a period of 5 days. The cytotoxicity of these cells was tested on 51Cr-labelled autologous dissociated glioblastoma cells which had not been cultured. Significant cytotoxicity against glioma cells was observed in seven out of nine cases. IL-2 activated PBL from normal donors were equally cytotoxic against these glioma cells. Autologous lymphocytes activated by phytohaemagglutinin were also lysed in most cases, and the erythroleukemia cell line K562 was highly susceptible to the cytotoxic capability of the IL-2 activated PBL. In cold target inhibition experiments, K562 inhibited the cytotoxicity against both autologous and allogenic glioma cells, and glioma cells inhibited the cytotoxicity against K562. Following immunomagnetic separation, the IL-2 activated cells demonstrated cytotoxicity against glioma cells, K562 cells, and PHA blasts in both the CD8+ and the CD8- subsets.
我们将胶质母细胞瘤患者的外周血淋巴细胞（PBL）在含有重组白细胞介素-2（IL-2）的培养基中培养5天。用未培养过的51Cr标记的自体解离胶质母细胞瘤细胞检测这些细胞的细胞毒性。在9例中有7例观察到对胶质瘤细胞有显著的细胞毒性。来自正常供体的IL-2激活的PBL对这些胶质瘤细胞同样具有细胞毒性。在大多数情况下，经植物血凝素激活的自体淋巴细胞也会被裂解，并且红白血病细胞系K562对IL-2激活的PBL的细胞毒性高度敏感。在冷靶抑制实验中，K562抑制对自体和同种异体胶质瘤细胞的细胞毒性，而胶质瘤细胞抑制对K562的细胞毒性。免疫磁珠分离后，IL-2激活的细胞在CD8 +和CD8 -亚群中均表现出对胶质瘤细胞、K562细胞和PHA刺激的淋巴细胞的细胞毒性。
