Trapani J A, Klein J L, White P C, Dupont B
Laboratory of Human Immunogenetics, Memorial Sloan-Kettering Cancer Center, New York, NY 10021.
Proc Natl Acad Sci U S A. 1988 Sep;85(18):6924-8. doi: 10.1073/pnas.85.18.6924.
A cDNA clone encoding a human serine esterase gene was isolated from a library constructed from poly(A)+ RNA of allogeneically stimulated, interleukin 2-expanded peripheral blood mononuclear cells. The clone, designated HSE26.1, represents a full-length copy of a 0.9-kilobase mRNA present in human cytotoxic cells but absent from a wide variety of noncytotoxic cell lines. Clone HSE26.1 contains an 892-base-pair sequence, including a single 741-base-pair open reading frame encoding a putative 247-residue polypeptide. The first 20 amino acids of the polypeptide form a leader sequence. The mature protein is predicted to have an unglycosylated Mr of approximately equal to 26,000 and contains a single potential site for N-linked glycosylation. The nucleotide and predicted amino acid sequences of clone HSE26.1 are homologous with all murine and human serine esterases cloned thus far but are most similar to mouse granzyme B (70% nucleotide and 68% amino acid identity). HSE26.1 protein is expressed weakly in unstimulated peripheral blood mononuclear cells but is strongly induced within 6-hr incubation in medium containing phytohemagglutinin. The data suggest that the protein encoded by HSE26.1 plays a role in cell-mediated cytotoxicity.
从一个由同种异体刺激、白细胞介素2扩增的外周血单个核细胞的聚腺苷酸加尾RNA构建的文库中分离出一个编码人丝氨酸酯酶基因的cDNA克隆。该克隆命名为HSE26.1，代表一种0.9千碱基mRNA的全长拷贝，此mRNA存在于人类细胞毒性细胞中，但在多种非细胞毒性细胞系中不存在。克隆HSE26.1包含一个892个碱基对的序列，包括一个单一的741个碱基对的开放阅读框，编码一个推定的247个残基的多肽。该多肽的前20个氨基酸形成一个前导序列。预测成熟蛋白的未糖基化分子量约为26,000，并且含有一个单一的N-连接糖基化潜在位点。克隆HSE26.1的核苷酸和预测的氨基酸序列与迄今克隆的所有鼠类和人类丝氨酸酯酶同源，但与小鼠颗粒酶B最为相似（核苷酸同一性为70%，氨基酸同一性为68%）。HSE26.1蛋白在未刺激的外周血单个核细胞中弱表达，但在含有植物血凝素的培养基中孵育6小时内被强烈诱导。数据表明，HSE26.1编码的蛋白在细胞介导的细胞毒性中起作用。
