Silva Leandro Maria Karollyna do N, Rocha Janaína E, Bezerra Camila F, Freitas Priscilla R, Feitosa Jessica Horana F, Bezerra Viviane B, Barros Rizelle de O, Leandro Livia Maria G, Aguiar José Junior Dos S, Pereira Paulo S, Christofoli Marcela, Ribeiro-Filho Jaime, Iriti Marcello, Coutinho Henrique Douglas M, Matias Edinardo Fagner F
Laboratory of Microbiology and Molecular Biology - LMBM, Regional University of Cariri- URCA, Crato, Ceara, Brazil.
Leão Sampaio University Center - Unileão, Juazeiro do Norte, Ceara, Brazil.
Z Naturforsch C J Biosci. 2020 Nov 26;75(11-12):377-387. doi: 10.1515/znc-2020-0034.
This study aimed to evaluate the antibacterial and antibiotic-enhancing effects of the essential oil obtained from Ocimum gratissimum L. (OEOg) alone or in association with light-emitting diodes (LED) lights. The essential oil was obtained by hydrodistillation and its chemical composition analysed by gas chromatography coupled to mass spectrometry. The antibacterial and antibiotic-enhancing activities against multiresistant strains of Staphylococcus aureus and Escherichia coli were evaluated by the gaseous contact method. The analysis of the photoinductive effect on the antibacterial activity of the OEOg and antibiotics was assessed through exposure to different LED lights (red, blue and yellow). The phytochemical analysis identified five compounds, including eugenol, as the major constituent. The OEOg caused a significant inhibition of the halo, indicating a direct antibacterial effect. Exposure to the LED lights significantly enhanced the activity of the OEOg against E. coli. On the other hand, the action of the essential oil against S. aureus was enhanced by exposure to both blue and yellow lights. The effects of LED light exposure on the activity of conventional antibiotics varied significantly according to the drug and the bacterial strain. However, most combinations of LED lights and the OEOg presented synergistic effects against resistant bacterial strains, indicating enhanced antibacterial activity. Thus, these in vitro findings suggest that both OEOg and LED lights have promising antibacterial effects. Nevertheless, further research is required to evaluate in vivo the potential of these therapies for the treatment of infectious skin diseases.
本研究旨在评估单独使用或与发光二极管（LED）灯联合使用时，从丁香罗勒（Ocimum gratissimum L.）中提取的精油（OEOg）的抗菌及增强抗生素效果。通过水蒸馏法获得精油，并采用气相色谱-质谱联用仪分析其化学成分。采用气体接触法评估对金黄色葡萄球菌和大肠杆菌多重耐药菌株的抗菌及增强抗生素活性。通过暴露于不同的LED灯（红色、蓝色和黄色）来评估光诱导对OEOg和抗生素抗菌活性的影响。植物化学分析鉴定出五种化合物，其中丁香酚为主要成分。OEOg对抑菌圈有显著抑制作用，表明具有直接抗菌效果。暴露于LED灯下显著增强了OEOg对大肠杆菌的活性。另一方面，暴露于蓝光和黄光下增强了精油对金黄色葡萄球菌的作用。根据药物和细菌菌株的不同，LED灯照射对传统抗生素活性的影响差异显著。然而，大多数LED灯与OEOg的组合对耐药菌株呈现协同作用，表明抗菌活性增强。因此，这些体外研究结果表明，OEOg和LED灯均具有良好的抗菌效果。尽管如此，仍需要进一步研究以在体内评估这些疗法治疗感染性皮肤病的潜力。
