Studies and MAO Inhibitory Activity of Coumarins Isolated from Extract: An Approach toward Antidepressant Activity.

The current investigation was aimed at MAO inhibitory activity of coumarins angelicin, bergapten, and scopoletin isolated from the roots of . The isolated compounds were screened for MAO (pdb ID 2Z5y) binding through molecular docking studies. The molecular docking results displayed that bergapten has a maximum affinity for MAO, followed by angelicin and scopoletin. prediction of physicochemical parameters indicated that maximum blood-brain barrier (BBB) permeability was observed with angelicin (2.3), followed by bergapten (2.0) and least with scopoletin (0.644). In consonance to the results of molecular docking studies, appreciable antidepressant activity of angelicin and bergaptan was observed over the mouse model of reserpine-induced depression. The modulation of MAO in the antidepressant effect of extract and its isolated fractions was also determined. Biochemical examination of the brain tissue indicated that bergapten has maximum MAO inhibitory activity while scopoletin fails to inhibit brain MAO.