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在拉伸超过肌动蛋白和肌球蛋白丝重叠范围后释放的去皮肌纤维中肌动蛋白丝的定位及张力产生

Positioning of actin filaments and tension generation in skinned muscle fibres released after stretch beyond overlap of the actin and myosin filaments.

作者信息

Higuchi H, Yoshioka T, Maruyama K

机构信息

Department of Physiology, Jikei University School of Medicine, Tokyo, Japan.

出版信息

J Muscle Res Cell Motil. 1988 Dec;9(6):491-8. doi: 10.1007/BF01738754.

DOI:10.1007/BF01738754
PMID:3264837
Abstract

Skinned fibres from frog semitendinosus muscle were stretched in relaxing solution from a sarcomere length of 2.5 microns to greater sarcomere lengths, and then shortened back to the original length. Fibres could be stretched up to sarcomere lengths of 3.3 microns, and reshortened fully. If the original stretch was to a sarcomere length greater than 3.3 microns, the extent of recovery was dependent on the magnitude of the stretch and the number of times the stretch/shorten cycle was repeated. When the original stretch was to sarcomere lengths beyond overlap of the thick and thin filaments, the thin filaments did not re-enter the thick filament array but buckled at the A-I junction. If these fibres were subsequently activated and contracted, the thin filaments re-entered the thick filament array, taking up approximately their former positions, and allowing reduced development of isometric tension.

摘要

将青蛙半腱肌的去皮肤肌纤维在松弛溶液中从2.5微米的肌节长度拉伸至更长的肌节长度,然后再缩短回到原始长度。肌纤维可以被拉伸至3.3微米的肌节长度,并完全重新缩短。如果最初的拉伸至大于3.3微米的肌节长度,恢复程度取决于拉伸的幅度以及拉伸/缩短循环重复的次数。当最初的拉伸至粗、细肌丝重叠范围之外的肌节长度时,细肌丝不会重新进入粗肌丝阵列,而是在A-I连接处弯曲。如果这些肌纤维随后被激活并收缩,细肌丝会重新进入粗肌丝阵列,占据大致原来的位置,并使得等长张力的发展减弱。

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The effect of calcium activation of skinned fiber bundles on the structure of Limulus thick filaments.皮膜纤维束的钙激活对鲎粗肌丝结构的影响。
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