维生素D代谢物高压液相色谱中的氚同位素效应

Tritium isotope effect in high-pressure liquid chromatography of vitamin D metabolites.

作者信息

Worth G K, Retallack R W

机构信息

Department of Endocrinology and Diabetes, Sir Charles Gairdner Hospital, Nedlands, Western Australia.

出版信息

Anal Biochem. 1988 Oct;174(1):137-41. doi: 10.1016/0003-2697(88)90527-1.

DOI:10.1016/0003-2697(88)90527-1

PMID:3265289

Abstract

A significant chromatographic isotope effect is reported for 1,25-dihydroxyvitamin D3 in a wide variety of HPLC separation systems. The effect is also observed for 24,25-dihydroxyvitamin D3. Retention times differ from less than 1% up to 4% depending on the separation system and the degree and position of tritium substitution. Such an effect must be corrected for whenever both labeled and unlabeled vitamin D metabolites are used in HPLC cochromatography or assay recovery studies.

摘要

在多种高效液相色谱（HPLC）分离系统中，均报道了1,25 - 二羟基维生素D3存在显著的色谱同位素效应。在24,25 - 二羟基维生素D3中也观察到了这种效应。保留时间的差异从不到1%到4%不等，这取决于分离系统以及氚取代的程度和位置。每当在HPLC共色谱分析或测定回收率研究中同时使用标记和未标记的维生素D代谢物时，都必须对这种效应进行校正。