Salinity alleviates the arsenic toxicity in the facultative halophyte Salvadora persica L. by the modulations of physiological, biochemical, and ROS scavenging attributes.

Heavy metal(loid)s contamination in soil is a major environmental concern that limits agricultural yield and threatens human health worldwide. Arsenic (As) is the most toxic non-essential metalloid found in soil which comes from various natural as well as human activities. S. persica is a facultative halophyte found abundantly in dry, semiarid and saline areas. In the present study, growth, mineral nutrient homeostasis, MDA content, phytochelatin levels, and ROS-scavenging attributes were examined in S. persica imposed to solitary treatments of salinity (250 mM and 750 mM NaCl), solitary treatments of arsenic (200 μM and 600 μM As), and combined treatments of As with 250 mM NaCl with an aim to elucidate salinity and As tolerance mechanisms. The results demonstrated that S. persica plants sustained under high levels of As (600 μM As) as well as NaCl (750 mM). The activity of superoxide dismutase, catalase, peroxidase, and glutathione reductase were either elevated or unaffected under salt or As stress. However, ascorbate peroxidase activity declined under both solitary and combination of As with NaCl. Furthermore, the cellular redox status measured in terms of reduced ascorbate/dehydroascorbate, and reduced glutathione/oxidized glutathione ratios also either increased or remained unaffected in seedlings treated with both solitary and combined treatments of As + NaCl. Significant accumulation of various oxidative stress indicators (HO and O were observed under high As stress condition. However, presence of salt with high As significantly reduced the levels of ROS. Furthermore, exogenous salt improved As tolerance index (Ti) under high As stress condition. The values of translocation factor (Tf) and As bioaccumulation factor (BF) were >1 in all the treatments. From this study, it can be concluded that the facultative halophyte S. persica is a potential As accumulator and may find application for phytoextraction of arsenic-contaminated saline soil.