Lahagu Eunike Amelina, Fachiroh Jajah, Anugrah Andreas Surya, Gunawan Wasisdi, Mahayana Indra Tri
Department of Ophthalmology, Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada-Dr.Sardjito General Hospital, Yogyakarta 55284, Indonesia.
Department of Biomolecular and Cell Biology, Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada, Yogyakarta 55284, Indonesia.
Int J Ophthalmol. 2020 Jul 18;13(7):1148-1151. doi: 10.18240/ijo.2020.07.19. eCollection 2020.
To investigate the changes in levels of the lactate dehydrogenase (LDH) enzyme in corneal edema after cataract surgery with trans-corneal oxygenation therapy.
This pre-post design study design conducted on 15 patients with corneal edema after cataract surgery and receiving trans-corneal oxygenation therapy. Tear sample (using Schirmer paper, from the inferior fornix of the conjunctiva) was carried out prior to trans-corneal oxygenation therapy, on the day 2 (D2) and day 5 (D5) postoperatively before and after trans-corneal oxygenation therapy. Visual acuity [VA (LogMAR)], corneal endothelial density, central corneal thickness (CCT), and coefficient of variation corneal endothelial (CoV) were recorded. The value of LDH was measured using ELISA. The difference in mean LDH value before and after trans-corneal oxygenation therapy, between two groups were analyzed using Wilcoxon signed rank test.
There was a decrease in LDH tear concentration at D2 (pre post: 1127.54±497.09 696.91±489.49; =0.002) and D5 (pre post: 1064.17±677.77 780.28±428.95; =0.027) after trans-corneal oxygenation therapy as well as decrease in LDH concentration on the D2 compared to D5 (=0.041). The mean CCT was decreased significantly after the administration of trans-corneal oxygenation (pre post: 632.10±25.66 563.90±51.54; =0.005). The mean VA and CoV increased significantly after the administration of trans-corneal oxygenation (=0.001 and =0.028, respectively). However, there was no difference in mean of corneal endothelial density (=0.814).
Trans-corneal oxygenation therapy is associated with significant decrease of tears LDH levels in post cataract surgery with corneal edema. It is accompanied by clinical improvement such as significant reduction of CCT.
研究经角膜氧合治疗白内障手术后角膜水肿中乳酸脱氢酶（LDH）水平的变化。
本前后对照研究设计针对15例白内障手术后角膜水肿并接受经角膜氧合治疗的患者进行。在经角膜氧合治疗前、术后第2天（D2）和第5天（D5），使用泪液试纸从结膜下穹窿采集泪液样本。记录视力[VA（LogMAR）]、角膜内皮细胞密度、中央角膜厚度（CCT）和角膜内皮变异系数（CoV）。使用酶联免疫吸附测定法（ELISA）测量LDH值。采用Wilcoxon符号秩检验分析经角膜氧合治疗前后两组LDH平均值得差异。
经角膜氧合治疗后，D2（治疗前 治疗后：1127.54±497.09 696.91±489.49；P = 0.002）和D5（治疗前 治疗后：1064.17±677.77 780.28±428.95；P = 0.027）时泪液中LDH浓度降低，且D2时的LDH浓度低于D5（P = 0.041）。给予经角膜氧合治疗后，平均CCT显著降低（治疗前 治疗后：632.10±25.66 563.90±51.54；P = 0.005）。给予经角膜氧合治疗后，平均VA和CoV显著增加（分别为P = 0.001和P = 0.028）。然而，角膜内皮细胞密度平均值无差异（P = 0.814）。
经角膜氧合治疗与白内障手术后角膜水肿患者泪液LDH水平显著降低相关。同时伴有临床改善，如CCT显著降低。
