GonÇalves FabÍola V, Medici Leonardo O, Fonseca Marcos Paulo S DA, Pimentel Carlos, Gaziola Salete A, Azevedo Ricardo A
Universidade Federal Rural do Rio de Janeiro, Departamento de Fitotecnia - IA, BR 465, Km 47, 23897-000 Seropédica, RJ, Brazil.
Universidade Federal Rural do Rio de Janeiro, Departamento de Ciências Fisiológicas - ICBS, BR 465, Km 47, 23897-000 Seropédica, RJ, Brazil.
An Acad Bras Cienc. 2020 Jul 29;92(suppl 1):e20180484. doi: 10.1590/0001-3765202020180484.
The objective of this study was to investigate and characterize cowpea (Vigna unguiculata) genotypes for total grain protein content, storage protein fractions (globulin, albumin, prolamin, basic and acid glutelins), and phytate and minerals contents. Eighteen cowpea genotypes were selected. Total grain protein content varied from 21.4% to 29.2%, for BRS Marataoã and Paulistinha genotypes, respectively. The variation in the concentration of each protein fraction was significant (P<0.05) only for glutelins (basic and acid). The genotypes studied exhibited great similarity in the PAGE electrophoretic profile of the grain protein fractions and also in the mineral content. BRS Paraguaçu genotype exhibited higher Zn content than thegenotypes that have been previously recommended for this characteristic. The lowest phytate grain content was observed in four of the 18 genotypes studied, which also exhibited high protein contents. Although the results did not converge to the selection of a few genotypes, some specific differences were detected that which may be further explored. Considering total grain protein, mineral and phytate contents, the genotype Paulistinha revealed a better balance unveiling high grain total protein content, low grain phytate content and more homogeneous mineral composition.
本研究的目的是调查和表征豇豆(Vigna unguiculata)基因型的总籽粒蛋白含量、贮藏蛋白组分(球蛋白、清蛋白、醇溶蛋白、碱性和酸性谷蛋白)以及植酸盐和矿物质含量。选取了18个豇豆基因型。BRS Marataoã和Paulistinha基因型的总籽粒蛋白含量分别在21.4%至29.2%之间。仅谷蛋白(碱性和酸性)的各蛋白组分浓度变化具有显著性(P<0.05)。所研究的基因型在籽粒蛋白组分的PAGE电泳图谱以及矿物质含量方面表现出高度相似性。BRS Paraguaçu基因型的锌含量高于先前因该特性而被推荐的基因型。在所研究的18个基因型中有4个的籽粒植酸盐含量最低,这些基因型的蛋白含量也很高。尽管结果并未集中于少数基因型的选择,但检测到了一些可能有待进一步探索的特定差异。综合考虑总籽粒蛋白、矿物质和植酸盐含量,Paulistinha基因型表现出更好的平衡,具有较高的籽粒总蛋白含量、较低的籽粒植酸盐含量以及更均匀的矿物质组成。