Grabowska M, Szala S, Chorazy M
Acta Biochim Pol. 1977;24(2):143-51.
Two fractions of rat liver DNA: "intermediate" (reassociated to COt = 1 - 10(2), moleXsecX1(-1)) and "slow" (COt = 10(2) - 10(5) showed differences in template activity on transcription with E. coli RNA-polymerase. The "intermediate" fraction both in double- and single-stranded form was a better template than the "slow" one. The efficiency of transcription dropped progressively for the template obtained at COt values increasing from 10(2) to 10(6). No differences in the template activity of the "intermediate" and "slow" fractions were observed when rifampicin-resistant transcription was studied.
大鼠肝脏DNA的两个组分：“中等组分”（复性至COt = 1 - 10²，摩尔×秒×升⁻¹）和“慢组分”（COt = 10² - 10⁵）在与大肠杆菌RNA聚合酶转录时的模板活性上表现出差异。“中等组分”无论是双链还是单链形式，都是比“慢组分”更好的模板。对于在COt值从10²增加到10⁶时获得的模板，转录效率逐渐下降。在研究利福平抗性转录时，未观察到“中等组分”和“慢组分”的模板活性有差异。
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