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用于研究B淋巴细胞活化的牵引力显微镜技术

Traction Force Microscopy to Study B Lymphocyte Activation.

作者信息

Kumari Anita, Pineau Judith, Lennon-Duménil Ana-Maria, Balland Martial, Pierobon Paolo

机构信息

Institut Curie, PSL Research University, INSERM U932.

Laboratoire Interdisciplinaire de Physique, Université Joseph Fourier (Grenoble 1).

出版信息

J Vis Exp. 2020 Jul 23(161). doi: 10.3791/60947.

Abstract

Traction force microscopy (TFM) enables the measurement of forces produced by a cell on a substrate. This technique infers traction force measurements from an experimentally observed displacement field produced by a cell pulling on an elastic substrate. Here, we adapted TFM to investigate the spatial and temporal structure of the force field exerted by B cells when activated by antigen engagement of the B cell receptor. Gel rigidity, bead density, and protein functionalization must be optimized for the study of relatively small cells (~ 6 µm) that interact with, and respond specifically to ligands for cell surface receptors.

摘要

牵引力显微镜技术(TFM)能够测量细胞在基质上产生的力。该技术从细胞牵拉弹性基质所产生的实验观察到的位移场推断牵引力测量值。在此,我们采用TFM来研究B细胞受体的抗原结合激活B细胞时所施加的力场的空间和时间结构。对于研究与细胞表面受体配体相互作用并对其产生特异性反应的相对较小的细胞(约6微米),必须优化凝胶硬度、珠子密度和蛋白质功能化。

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