采用经过验证的高效液相色谱法快速灵敏地提取并测定其主要多酚化合物的方法。
Rapid and Sensitive Method for Extraction of with Determination of Its Main Polyphenolic Compounds Using Validated HPLC.
作者信息
AlZain Mashail N, Herqash Rashed N, Almoqbil Abdulaziz N, Mohammed Almarfadi Omer, Ibrahim Mansour N, Ullah Riaz, Noman Omar M
机构信息
Department of Biology, College of Sciences, Princess Nourah Bint Abdulrahman University, Riyadh 11451, Saudi Arabia.
Medicinal, Aromatic, and Poisonous Plants Research Center, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.
出版信息
J Anal Methods Chem. 2020 Jul 24;2020:9598606. doi: 10.1155/2020/9598606. eCollection 2020.
Matrix solid phase dispersion (MSPD) trailed by HPLC is a quick and fruitful technique utilized for fortitude of flavonoids such as Catechin, Kaempferol, Quercetin, and Rutin existing in . . The trial parameters that influenced the extraction potential (comprising the mass ratio of sample to the dispersant, nature of dispersant, and the nature of elution solvent and its volume) were examined and optimized. These MSPD optimized parameters regulated are as follows: 8 mL of methanol was utilized as elution solvent, silica gel/sample mass ration was selected to be 2 : 1, and dispersing sorbent was silica gel. The technique retrievals were regulated to be "from 96.87 to 100.54%" and the RSDs from 1.24 to 4.45%. The product of extract obtained by MSPD method was larger than that of other methods, i.e., sonication extraction or traditional reflux with lessened necessities on time, sample, and solvent.
高效液相色谱法（HPLC）联用基质固相分散法（MSPD）是一种快速且有效的技术，用于测定[具体物质]中存在的儿茶素、山奈酚、槲皮素和芦丁等黄酮类化合物的含量。研究并优化了影响提取效率的试验参数（包括样品与分散剂的质量比、分散剂的性质、洗脱溶剂的性质及其体积）。这些优化后的MSPD参数如下：使用8毫升甲醇作为洗脱溶剂，硅胶与样品的质量比选择为2:1，分散吸附剂为硅胶。该技术的回收率在96.87%至100.54%之间，相对标准偏差（RSD）在1.24%至4.45%之间。MSPD法获得的提取物产量高于其他方法，即超声提取或传统回流法，且对时间、样品和溶剂的需求减少。
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