Key Laboratory of Genetics and Fruit Development, Horticultural College, Nanjing Agricultural University, Nanjing, China.
Key Laboratory of Genetics and Fruit Development, Horticultural College, Nanjing Agricultural University, Nanjing, China.
Food Chem. 2021 Feb 1;337:127772. doi: 10.1016/j.foodchem.2020.127772. Epub 2020 Aug 4.
Chitosan can function a key role in plant resistant against Botrytis cinerea infection, while its mechanism is unclear in ripened fruits. In this study, we investigated the chitosan effect on two type of ripened fruits including strawberry and grapes (Kyoho and Shine-Muscat) when were infected with B. cinerea. Results showed that chitosan inhibited B. cinerea growth, increased phenolic compounds and cell wall composition, modulated oxidative stress and induced jasmonic acid (JA) production in ripened fruits. Data-independent acquisition (DIA) showed that 224 and 171 proteins were upregulated 1.5-fold by chitosan in Kyoho and Shine-Muscat grape, respectively. Topless-related protein 3 (TPR3) were identified and interacted with histone deacetylase 19 (HDAC19) and negatively regulated by JA and chitosan. Meanwhile, overexpression of VvTPR3 and VvHDAC19 reduced the stability of cell wall against B. cinerea in strawberry. Taken together, chitosan induces defense related genes and protect the fruit quality against Botrytis infection through JA signaling.
壳聚糖在植物抵抗灰葡萄孢菌感染方面可以发挥关键作用，但其在成熟果实中的作用机制尚不清楚。在本研究中，我们研究了壳聚糖对草莓和两种成熟葡萄（京秀和阳光玫瑰）感染灰葡萄孢菌的影响。结果表明，壳聚糖抑制了灰葡萄孢菌的生长，增加了酚类化合物和细胞壁成分，调节了氧化应激，并诱导了成熟果实中茉莉酸（JA）的产生。数据非依赖性采集（DIA）显示，壳聚糖分别使京秀和阳光玫瑰葡萄中的 224 和 171 种蛋白质上调了 1.5 倍。鉴定到与类 TOPLESS 蛋白 3（TPR3）相关的蛋白，并与组蛋白去乙酰化酶 19（HDAC19）相互作用，且受 JA 和壳聚糖的负调控。同时，过表达 VvTPR3 和 VvHDAC19 降低了草莓细胞壁对灰葡萄孢菌的稳定性。综上所述，壳聚糖通过 JA 信号诱导防御相关基因，并保护果实品质免受灰葡萄孢菌感染。
