Ottesen S S, Tromholt V, Kieler J
Fibiger Institute, Danish Cancer Society, Copenhagen, Denmark.
Cancer Immunol Immunother. 1988;26(1):83-6. doi: 10.1007/BF00199852.
Quantitative changes in major histocompatibility class I antigen expression in tumour cells are believed to affect the host immune response against the tumour. In tumourigenic (TGrIII) human urothelial cell lines the apparent loss of polymorphic HLA-A,B epitopes has previously been demonstrated. In the present study, 3 non-tumourigenic (TGrII) and 6 tumourigenic (TGrIII) human urothelial cell lines have been investigated for their quantitative expression of monomorphic HLA-A,B,C and B2-microglobulin. Evidence is provided that an inverse correlation exists between tumourigenicity and HLA-A,B,C and B2-microglobulin expression. Furthermore, treatment of the cells with neuraminidase partly restored the expression of monomorphic HLA-A,B,C suggesting that at least some of the observed quantitative differences could be due to masking of the membrane bound HLA antigens by sialic acid-containing glycoconjugates.
肿瘤细胞中主要组织相容性复合体I类抗原表达的定量变化被认为会影响宿主对肿瘤的免疫反应。在致瘤性(TGrIII)人膀胱上皮细胞系中,先前已证实多态性HLA - A、B表位明显缺失。在本研究中,对3种非致瘤性(TGrII)和6种致瘤性(TGrIII)人膀胱上皮细胞系的单态性HLA - A、B、C和β2 - 微球蛋白的定量表达进行了研究。有证据表明致瘤性与HLA - A、B、C和β2 - 微球蛋白表达之间存在负相关。此外,用神经氨酸酶处理细胞部分恢复了单态性HLA - A、B、C的表达,这表明至少一些观察到的定量差异可能是由于含唾液酸的糖缀合物对膜结合HLA抗原的掩盖所致。