Department of Chemistry and Chemical Engineering, Physical Chemistry, Chalmers University of Technology, SE-412 96 Gothenburg, Sweden.
Universidad de Málaga-IBIMA, Departamento de Quı́mica Orgánica, Campus Teatinos s/n, E-29071 Málaga, Spain.
J Am Chem Soc. 2020 Sep 2;142(35):14854-14858. doi: 10.1021/jacs.0c07377. Epub 2020 Aug 21.
The combination of two two-photon-induced processes in a Förster resonance energy transfer (FRET)-operated photochromic fluorene-dithienylethene dyad lays the foundation for the observation of a quartic dependence of the fluorescence signal on the excitation light intensity. While this photophysical behavior is predicted for a four-photon absorbing dye, the herein proposed approach opens the way to use two-photon absorbing dyes, reaching the same performance. Hence, the spatial resolution limit, being a critical parameter for applications in fluorescence imaging or data storage with common two-photon absorbing dyes, is dramatically improved.
在Förster 共振能量转移 (FRET) 作用下的光致变色芴-二噻吩乙烯偶联物中的两种双光子诱导过程的组合为观察荧光信号对激发光强度的四次依赖关系奠定了基础。虽然这种光物理行为是针对四光子吸收染料预测的，但这里提出的方法为使用双光子吸收染料开辟了道路，从而达到相同的性能。因此，空间分辨率极限是荧光成像或使用常见双光子吸收染料进行数据存储应用的关键参数，得到了显著提高。
