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通过羧酸共聚物配体实现单分散和水溶近红外二区量子点用于 SWIR 成像。

Monodisperse and Water-Soluble Quantum Dots for SWIR Imaging via Carboxylic Acid Copolymer Ligands.

机构信息

Department of Materials Science and Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States.

Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States.

出版信息

ACS Appl Mater Interfaces. 2020 Aug 12;12(32):35845-35855. doi: 10.1021/acsami.0c08255. Epub 2020 Jul 30.

Abstract

Compared to the visible and near-infrared, the short-wave infrared region (SWIR; 1000-2000 nm) has excellent properties for imaging: low autofluorescence, reduced scattering, and a low-absorption cross-section of blood or tissue. However, the general adoption of SWIR imaging in biomedical research will be enhanced by a broader availability of versatile and bright contrast materials. Quantum dots (QDs) are bright and compact SWIR emitters with narrow size distributions and emission spectra, but their use is limited by the shortcomings of established ligand systems for SWIR QDs. Established ligands often result in SWIR probes with either limited colloidal stability, large size, or broad size distribution or a combination of all three. We present a polymeric QD ligand designed to be compatible with oleate-coated QDs. Our polymeric acid ligand is a copolymer bearing carboxylic acid anchoring groups and PEG-550 chains to solubilize the QD-ligand construct. After a mild and rapid ligand exchange, the resulting constructs are compact (<11 nm hydrodynamic diameter) and have narrow size distribution. Both qualities are preserved for several months in isotonic saline. The constructs are bright , and to demonstrate their suitability for imaging, we perform whole-body imaging and lymphatic imaging, including visualization of lymphatic flow.

摘要

与可见近红外光相比,短波近红外光(SWIR;1000-2000nm)在成像方面具有极好的特性:自发荧光低、散射少、血液或组织的吸收截面低。然而,广泛应用于生物医学研究的 SWIR 成像将通过更广泛的多功能和明亮的对比材料得到增强。量子点(QDs)是明亮紧凑的 SWIR 发射器,具有狭窄的尺寸分布和发射光谱,但由于 SWIR QD 现有配体系统的缺点,其应用受到限制。现有的配体通常会导致 SWIR 探针的胶体稳定性有限、尺寸较大或分布较宽,或者三者兼有。我们提出了一种与油酸盐涂层 QD 兼容的聚合物 QD 配体。我们的聚合物酸配体是一种带有羧酸锚固基团和 PEG-550 链的共聚物,可溶解 QD-配体结构。在温和快速的配体交换后,得到的结构紧凑(<11nm 水动力直径),尺寸分布狭窄。在等渗盐水中保存几个月后仍保持这两种特性。这些结构很亮,为了证明它们在成像中的适用性,我们进行了全身成像和淋巴成像,包括淋巴流动的可视化。

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