Department of Crop Sciences, University of Illinois at Urbana-Champaign, IL 61801, U.S.A.
Plant Pathology and Plant-Microbe Biology Section, Cornell University, Geneva, NY 14456, U.S.A.
Mol Plant Microbe Interact. 2020 Nov;33(11):1277-1279. doi: 10.1094/MPMI-06-20-0158-A. Epub 2020 Oct 1.
causes fire blight, the most devastating bacterial disease of apples and pears in the United States and worldwide. The model strain Ea1189 has been extensively used to understand bacterial pathogenesis and molecular mechanisms of bacterial-plant interactions. In this work, we sequenced and assembled the de novo genome of Ea1189, using a combination of long Oxford Nanopore Technologies and short Illumina sequence reads. A complete gapless genome assembly of Ea1189 consists of a 3,797,741-bp circular chromosome and a 28,259-bp plasmid with 3,472 predicted genes, including 78 transfer RNAs, 22 ribosomal RNAs, and 20 noncoding RNAs. A comparison of the Ea1189 genome to previously sequenced complete genomes showed 99.94 to 99.97% sequence similarity with 314 to 946 single nucleotide polymorphisms. We believe that the availability of the complete genome sequence of strain Ea1189 will further support studies to understand evolution, diversity and structural variations of strains, as well as the molecular basis of pathogenesis and its interactions with host plants, thus facilitating the development of effective management strategies for this important disease.
导致火疫病，这是美国和全球范围内苹果和梨最具破坏性的细菌性疾病。模式菌株 Ea1189 被广泛用于了解细菌发病机制和细菌与植物相互作用的分子机制。在这项工作中，我们使用长 Oxford Nanopore Technologies 和短 Illumina 测序reads 的组合，对 Ea1189 进行了从头测序和组装。Ea1189 的完整无间隙基因组组装由 3,797,741-bp 环状染色体和一个 28,259-bp 质粒组成，其中包含 3,472 个预测基因，包括 78 个转移 RNA、22 个核糖体 RNA 和 20 个非编码 RNA。将 Ea1189 基因组与以前测序的完整基因组进行比较，显示与 314 到 946 个单核苷酸多态性的序列相似性为 99.94 到 99.97%。我们相信，Ea1189 菌株的完整基因组序列的可用性将进一步支持研究，以了解 菌株的进化、多样性和结构变异，以及发病机制及其与宿主植物相互作用的分子基础，从而促进这一重要疾病的有效管理策略的制定。
