胶束相分离-盐析萃取-超高效液相色谱-串联质谱法测定牛奶中的磺胺类药物
Determination of Sulfonamides in Milk by Cloud Point-Salting Out Extraction and Ultra-High-Performance Liquid Chromatography Tandem Mass Spectrometry.
机构信息
Xiangya School of Public Health, Central South University, Changsha, 410005, China.
Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Control and Prevention, Beijing, 100013, China.
出版信息
Anal Sci. 2020 Dec 10;36(12):1555-1559. doi: 10.2116/analsci.20N013. Epub 2020 Aug 21.
A method involving cloud point-salting out extraction (CPSOE) coupled with UHPLC-MS/MS was developed for the determination of eleven sulfonamides in milk. In this study, the type and concentration of the surfactant, de-emulsification condition, pH value, volume of n-butanol, equilibration temperature and time were optimized. For this developed method, the linear range of SAs was from 0.05 to 50 μg L, and the correlation coefficients were higher than 0.997. The average recoveries for SAs were from 61.32 to 91.67%, and the LOQs were less than 0.06 μg kg.
建立了一种基于浊点萃取-超高效液相色谱串联质谱法（UHPLC-MS/MS）同时测定牛奶中 11 种磺胺类药物残留的方法。本研究考察了表面活性剂的种类及浓度、破乳条件、pH 值、正丁醇体积、平衡温度和时间等条件。在优化的实验条件下，11 种磺胺类药物在 0.05-50 μg·L 范围内线性关系良好，相关系数均大于 0.997。方法的平均回收率为 61.32%-91.67%，检出限为 0.06-0.20 μg·kg。
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