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电针预处理通过 JAK/STAT 通路减轻大鼠自体原位肝移植后肠道损伤。

Electroacupuncture Pretreatment Attenuates Intestinal Injury after Autogenous Orthotopic Liver Transplantation in Rats via the JAK/STAT Pathway.

机构信息

Department of Anesthesiology, Tianjin First Center Hospital, Tianjin 300192, China.

出版信息

Oxid Med Cell Longev. 2020 Aug 3;2020:9187406. doi: 10.1155/2020/9187406. eCollection 2020.

DOI:10.1155/2020/9187406
PMID:32832009
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7424380/
Abstract

BACKGROUND

Liver transplantation induces self-injury and affects remote organs, such as the lung, kidney, and intestine. Postoperative intestinal dysfunction has been associated with prolonged hospitalization and affects a patient's health and quality of life. Electroacupuncture (EA) has been proven effective in multiple organ protection. However, the potential mechanism underlying the protective effects of EA on intestinal injury after liver transplantation remains unclear.

METHODS

After establishing an autogenous orthotopic liver transplantation (AOLT) model, we studied the effects of EA pretreatment on intestinal injury after AOLT. We used the JAK2-specific inhibitor AG490 to explore the underlying mechanism. Histological analysis and apoptosis assays were used to evaluate intestinal injury. Oxidative stress index and inflammatory response were also measured after AOLT. Furthermore, we detected the phosphorylation levels of JAK2, STAT1, and STAT3 by Western blot.

RESULTS

We found that pretreatment with EA alleviated intestinal injury after AOLT, as shown by HE staining and TUNEL methods. EA pretreatment inhibited the expressions of p-JAK2, p-STAT1, and p-STAT3 in the intestines after AOLT. Upon treatment with JAK2-specific inhibitor AG490, intestinal injury was balanced.

CONCLUSION

The data indicated EA pretreatment alleviated intestinal injury after AOLT by inhibiting the JAK/STAT signaling pathway. These results provide basic evidence to support the potential therapeutic efficacy of EA.

摘要

背景

肝移植会引发自身损伤，并影响到肺、肾和肠道等远隔器官。术后肠道功能障碍与住院时间延长有关，影响患者的健康和生活质量。电针（EA）已被证明在多器官保护方面有效。然而，EA 对肝移植后肠道损伤的保护作用的潜在机制尚不清楚。

方法

建立自体原位肝移植（AOLT）模型后，我们研究了 EA 预处理对 AOLT 后肠道损伤的影响。我们使用 JAK2 特异性抑制剂 AG490 来探讨潜在机制。采用组织学分析和凋亡检测评估肠道损伤。还在 AOLT 后测量氧化应激指数和炎症反应。此外，我们通过 Western blot 检测 JAK2、STAT1 和 STAT3 的磷酸化水平。

结果

我们发现 EA 预处理可减轻 AOLT 后肠道损伤，HE 染色和 TUNEL 方法均显示这一点。EA 预处理抑制了 AOLT 后肠道中 p-JAK2、p-STAT1 和 p-STAT3 的表达。用 JAK2 特异性抑制剂 AG490 处理后，肠道损伤得到平衡。

结论

数据表明，EA 预处理通过抑制 JAK/STAT 信号通路减轻 AOLT 后肠道损伤。这些结果为 EA 的潜在治疗效果提供了基础证据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8fb/7424380/1cd85a4a7c84/OMCL2020-9187406.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8fb/7424380/2bb4deb784bf/OMCL2020-9187406.001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8fb/7424380/1531ba1359c0/OMCL2020-9187406.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8fb/7424380/a16fc335f7f9/OMCL2020-9187406.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8fb/7424380/fe24cf86b28a/OMCL2020-9187406.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8fb/7424380/85a5089570e0/OMCL2020-9187406.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8fb/7424380/1a5d934f1f7c/OMCL2020-9187406.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8fb/7424380/1cd85a4a7c84/OMCL2020-9187406.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8fb/7424380/2bb4deb784bf/OMCL2020-9187406.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8fb/7424380/d605f554cbec/OMCL2020-9187406.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8fb/7424380/1531ba1359c0/OMCL2020-9187406.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8fb/7424380/a16fc335f7f9/OMCL2020-9187406.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8fb/7424380/fe24cf86b28a/OMCL2020-9187406.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8fb/7424380/85a5089570e0/OMCL2020-9187406.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8fb/7424380/1a5d934f1f7c/OMCL2020-9187406.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8fb/7424380/1cd85a4a7c84/OMCL2020-9187406.008.jpg

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