Li Chenchen, Hu Wei, Wang Jinchen, Song Xinjian, Xiong Xiaoxing, Liu Zhihong
Hubei Key Laboratory of Biological Resources Protection and Utilization, Hubei Minzu University, Enshi 445000, China.
Analyst. 2020 Sep 14;145(18):6125-6129. doi: 10.1039/d0an00824a.
In this study, a turn-on two-photon fluorescent probe (Lyso-TP-NO) for nitric oxide (NO) was developed. It was synthesized using 4-ethylamino-1,8-naphthalimide as the two-photon fluorophore and N-methylaniline moiety as the reaction site. The probe and fluorophore were tested under one- and two-photon modes. The fluorescence intensity of the system was enhanced 23.1-fold after reacting with NO in the one-photon mode. However, the maximal two-photon action cross-section value of 200 GM was obtained under excitation at 840 nm. The probe exhibits high selectivity and sensitivity over other reactive oxygen species (ROS) and reactive nitrogen species (RNS), with a detection limit as low as 3.3 nM. The two-photon fluorescence imaging of living cells and mouse brain tissues can capture inflammation-induced endogenous NO production in lysosomes during stroke occurrence.
在本研究中，开发了一种用于一氧化氮（NO）的开启型双光子荧光探针（Lyso-TP-NO）。它以4-乙氨基-1,8-萘二甲酰亚胺作为双光子荧光团，以N-甲基苯胺部分作为反应位点合成。该探针和荧光团在单光子和双光子模式下进行了测试。在单光子模式下与NO反应后，体系的荧光强度增强了23.1倍。然而，在840 nm激发下获得的最大双光子作用截面值为200 GM。该探针对其他活性氧（ROS）和活性氮（RNS）具有高选择性和灵敏度，检测限低至3.3 nM。活细胞和小鼠脑组织的双光子荧光成像可以捕捉中风发生期间溶酶体中炎症诱导的内源性NO生成。
