CD44 和 AREG 在 HPV 和 HPV 鳞状细胞癌中经选择性酪氨酸激酶抑制剂治疗后的表达模式。
Expression Patterns of CD44 and AREG Under Treatment With Selective Tyrosine Kinase Inhibitors in HPV and HPV Squamous Cell Carcinoma.
机构信息
Department of Otorhinolaryngology Head and Neck Surgery, University Hospital Essen, University Duisburg-Essen, Essen, Germany
Department of Otorhinolaryngology Head and Neck Surgery, University Hospital Mannheim, Medical Faculty Mannheim, University Heidelberg, Mannheim, Germany.
出版信息
Cancer Genomics Proteomics. 2020 Sep-Oct;17(5):579-585. doi: 10.21873/cgp.20214.
BACKGROUND
We investigated the expression patterns of cluster of differentiation (CD) 44 and amphiregulin (AREG), two signaling molecules essential for cell proliferation and differentiation, under the influence of selective tyrosine kinase inhibitors (TKIs) in human papillomavirus (HPV) and HPV squamous carcinoma cell lines.
MATERIALS AND METHODS
The protein expression of CD44 and AREG was determined by sandwich enzyme-linked immunosorbent assay in HPV cell lines UMSCC-11A and UMSCC-14C, and HPV CERV-196 cells after TKI treatment.
RESULTS
The expression of AREG and CD44 was dependent on the cell line's HPV status. AREG expression increased after incubation with nilotinib in HPV tumor cells. The expression of CD44 was significantly influenced by all drugs; its expression under selective epidermal growth factor receptor inhibition was mostly reduced, whereas nilotinib led to an exceptional increase of CD44 expression.
CONCLUSION
The selective drug treatment options significantly influenced the expression of CD44 and AREG in HPV and HPV tumor cells, constituting the need for personalized treatment options.
背景
我们研究了细胞分化（CD）44 和 Amphiregulin（AREG）这两种信号分子在人乳头瘤病毒（HPV）和 HPV 鳞状癌细胞系中受选择性酪氨酸激酶抑制剂（TKI）影响的表达模式。
材料和方法
采用夹心酶联免疫吸附试验检测 HPV 细胞系 UMSCC-11A 和 UMSCC-14C 以及 HPV CERV-196 细胞在 TKI 处理后的 CD44 和 AREG 蛋白表达。
结果
AREG 和 CD44 的表达取决于细胞系的 HPV 状态。在 HPV 肿瘤细胞中用尼罗替尼孵育后 AREG 表达增加。CD44 的表达受所有药物的显著影响；表皮生长因子受体选择性抑制下的 CD44 表达大多降低，而尼罗替尼导致 CD44 表达异常增加。
结论
选择性药物治疗方案显著影响 HPV 和 HPV 肿瘤细胞中 CD44 和 AREG 的表达，这构成了个性化治疗方案的需求。
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